Differentiation between spores of Bacillus anthracis and Bacillus cereus by a quantitative immunofluorescence technique.

Journal of Clinical Microbiology
A P PhillipsM G Broster

Abstract

A quantitative immunofluorescence assay based on fiber optic microscopy was used to measure the reaction of formalized spores of Bacillus anthracis and Bacillus cereus isolates with fluorescein conjugates prepared by hyperimmunization with B. anthracis Vollum spores. The spores of 11 of the 20 B. cereus strains reacted with the anti-anthrax conjugate to such an extent that they were indistinguishable from the spores of the several B. anthracis isolates tested. However, absorption of the conjugate with spores of B. cereus NCTC 8035 and B. cereus NCTC 10320 greatly reduced the cross-reaction with the B. cereus preparations so that the mean specific fluorescence of samples of B. cereus spores was in no case higher than 14% of the fluorescence of a reference B. anthracis Vollum preparation.

References

Mar 1, 1966·Applied Microbiology·A F WellsM K Nadel
Jan 1, 1971·Applied Microbiology·L H Angelety, G G Wright
Jan 1, 1981·Journal of Immunological Methods·D McCarthyE J Holborow
Jul 1, 1962·Journal of General Microbiology·J R NORRIS

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Citations

Dec 1, 1988·FEMS Microbiology Immunology·A P PhillipsR Quinn
Jan 1, 1990·Microbiology and Immunology·T MikamiM Suzuki
Apr 17, 2003·Journal of Food Protection·M C Erickson, J L Kornacki
Jan 1, 1992·The Journal of Applied Bacteriology·P C TurnbullJ Melling
May 1, 1989·The Journal of Applied Bacteriology·A P Phillips, J W Ezzell
Dec 1, 1983·Applied and Environmental Microbiology·A P Phillips, K L Martin
Jan 1, 1984·Journal of Clinical Microbiology·H B ColeR J Doyle
May 1, 1984·Journal of Clinical Microbiology·D F SadlerR J Doyle

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