PMID: 9536288Apr 16, 1998Paper

Differentiation-dependent and stimulus-specific expression of ILA, the human 4-1BB-homologue, in cells of mesenchymal origin

Osteoarthritis and Cartilage
J von KempisM Lotz

Abstract

Examine the expression of ILA, a member of the human NGF/TNF receptor family and the homologue of the murine 4-1BB, in mesenchymal cells. ILA mRNA was analyzed by quantitative polymerase chain reaction and Northern blotting in human articular chondrocytes and fibroblasts. ILA protein expression was examined by flow cytometry. The proinflammatory stimuli interleukin-1 beta (IL-1 beta), tumour necrosis factor (TNF) alpha, leukemia inhibitory factor (LIF), interferon (IFN) gamma and lipopolysaccharide (LPS) induced ILA mRNA in primary human articular chondrocytes. TGF beta and dexamethasone inhibited IL-1 induced ILA expression. Chondrocytes expressed the 4.8, 4.0 and 1.9 kb isoforms of ILA mRNA which had previously been observed in lymphocytes and additional isoforms at 3.2, 1.5 and 1.2 kb. Cycloheximide alone induced ILA mRNA in primary chondrocytes while the combination of IL-1 and cycloheximide resulted in ILA superinduction. In contrast to primary chondrocytes, activated human synovial or skin fibroblasts did not express ILA mRNA. Furthermore, ILA was no longer inducible by IL-1 in subcultured, dedifferentiated chondrocytes. However, repression of ILA in fibroblasts and dedifferentiated chondrocytes was overcome by cyclohexim...Continue Reading

References

May 7, 1992·Nature·R J ArmitageC R Maliszewski
Oct 17, 1991·Nature·J Banchereau, F Rousset
Apr 15, 1991·Biochemical and Biophysical Research Communications·C A SmithR G Goodwin
Nov 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·T KohnoJ L Vannice
Jan 1, 1989·Annual Review of Immunology·B Beutler, A Cerami
Dec 21, 1989·Nature·Y J LiuI C MacLennan
Mar 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·B S Kwon, S M Weissman
Nov 21, 1986·Cell·D JohnsonM Chao
Dec 1, 1982·Experimental Cell Research·M DuchêneP K Müller
Sep 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·G Q ZhaoB de Crombrugghe
Sep 1, 1994·European Journal of Immunology·M R AldersonW S Din
Jul 16, 1993·Science·S RabizadehD E Bredesen
Apr 1, 1993·Experimental Cell Research·M IwamotoM Pacifici
Sep 1, 1994·Osteoarthritis and Cartilage·J J RediskeM Lotz

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Citations

Apr 11, 2001·American Journal of Clinical Pathology·K BrollH Schwarz
Dec 4, 2003·International Journal of Cancer. Journal International Du Cancer·Carla PalmaCristina Goso
Jul 2, 2015·Nature Reviews. Drug Discovery·Sjoukje J C van der StegenMichel Sadelain
May 18, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Álvaro TeijeiraAna Rouzaut
May 21, 2020·International Journal of Molecular Sciences·Nico M SieversNiels Schaft
Dec 19, 2007·The Journal of Biological Chemistry·Jonathan B FitzgeraldAlan J Grodzinsky

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