PMID: 9419766Apr 1, 1994Paper

Differentiation-dependent expression of the BCL-2 proto-oncogene in the human trophoblast lineage

Journal of the Society for Gynecologic Investigation
N SakuragiJ F Strauss

Abstract

We explored the role of the BCL-2 proto-oncogene in the life cycle of trophoblast cells by examining: 1) the patterns of BCL-2 expression in normal placenta at various gestational ages and in specimens of hydatidiform moles and choriocarcinomas, and 2) the effects of cyclic adenosine monophosphate (cAMP) treatment of JEG-3 choriocarcinoma cells, which induces differentiated functions, on BCL-2. BCL-2 protein was localized by indirect immunofluorescence and immunoperoxidase staining of tissue sections and cells using monoclonal and polyclonal antibodies. Western and Northern blotting were used to assess BCL-2 and p53 protein and mRNA levels, respectively. JEG-3 cells were transfected with a BCL-2 expression plasmid to establish that BCL-2 protein could be expressed at high levels in this cell type. BCL-2 immunostaining was most prominent in the syncytiotrophoblast of normal placenta. It was found in syncytiotrophoblast of complete and partial hydatidiform moles, whereas cytotrophoblast staining was weak. BCL-2 immunostaining was also barely detectable in choriocarcinoma cells (JEG-3 cells) and a primary choriocarcinoma. However, BCL-2 protein could be transiently overexpressed in JEG-3 cells by transfection with an expression pl...Continue Reading

References

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Citations

May 4, 2000·American Journal of Physiology. Cell Physiology·R LevyD M Nelson
May 16, 1998·International Journal of Cancer. Journal International Du Cancer·N SakuragiS Fujimoto
Sep 8, 2004·Journal of the Society for Gynecologic Investigation·Berthold Huppertz, John C P Kingdom
Jun 19, 2007·Apoptosis : an International Journal on Programmed Cell Death·Sarah J MollAlexander E P Heazell
May 26, 2016·International Journal of Molecular Medicine·Junjie LuYi Tan
Jul 21, 2006·Annals of the New York Academy of Sciences·Nicoletta di SimoneAlessandro Caruso
Mar 20, 2002·American Journal of Reproductive Immunology : AJRI·S SavionV Toder
Jun 10, 1998·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J R Harris
May 10, 2001·The Journal of Clinical Endocrinology and Metabolism·O KurachiT Maruo
Jan 7, 2004·Journal of the Society for Gynecologic Investigation·D W BurleighT G Golos
Oct 9, 2020·American Journal of Obstetrics and Gynecology·Lawrence H LinKevin M Elias
Apr 4, 2006·American Journal of Obstetrics and Gynecology·Berthold HuppertzJohn C P Kingdom
Jan 2, 2010·Placenta·M Gauster, B Huppertz

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