Differentiation-dependent repression of c-myc, B22, COX II and COX IV genes in murine erythroleukemia (MEL) cells

Biochemical Pharmacology
Ioannis S VizirianakisAsterios S Tsiftsoglou

Abstract

Murine erythroleukemia (MEL) cells provide a valuable model system for uncovering the cellular and molecular mechanisms of differentiation of proerythroid cells in culture. In order to characterize genes and gene expression patterns unique for erythropoiesis, we: (i) cloned and sequenced a 226bp cDNA encoding portion of the 3'-end B22 subunit of mitochondrial NADH-ubiquinone oxidoreductase (complex I); (ii) assessed the steady state level of RNA transcripts encoded by B22, cytochrome c oxidase (COX II, COX IV) and c-myc genes in MEL cells undergoing terminal differentiation induced by dimethylsulfoxide (DMSO) and/or 2-(3-ethylureido)-6-methylpyridine; and (iii) investigated whether the gene expression patterns of B22, COX IV and c-myc genes seen in differentiating cells are affected by N(6)-methyladenosine, an inhibitor of commitment and RNA methylation. These studies have indicated: (a) c-myc, COX II and COX IV genes exhibited biphasic expression pattern; a transient accumulation of c-myc, COX II and COX IV mRNAs was followed by a decline after 36hr incubation with DMSO and/or 2-(3-ethylureido)-6-methylpyridine, (b) B22 gene expression declined progressively in differentiated cells, (c) blockade of differentiation of MEL cells...Continue Reading

References

Dec 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·A S TsiftsoglouS H Robinson
Sep 24, 1999·Biochemical and Biophysical Research Communications·K I MillsA K Burnett
Jan 5, 2001·Nucleic Acids Research·M SchuhmacherD Eick
Feb 27, 2001·FEBS Letters·S NasiL Soucek

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Citations

Dec 4, 2003·Pharmacology & Therapeutics·Asterios S TsiftsoglouIoannis S Vizirianakis
Nov 2, 2005·Journal of Cellular Physiology·Pascal SeyerChantal Wrutniak-Cabello

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