Differentiation dynamics of mammary epithelial stem cells from Korean holstein dairy cattle under ECM-free conditions

Journal of Biomolecular Structure & Dynamics
Neelesh SharmaDong Kee Jeong

Abstract

The "stem cells" are commonly defined as "cells capable of self-renewal through replication and differentiating into specific lineages". The mammary gland contains functional stem/progenitor cells. The current study was planned with the objectives to study the differentiation dynamics of Korean Holstein mammary epithelial stem cells (KHMESCs) under the optimum culture conditions. Lineage negative KHMESCs isolated from mammary tissue of lactating cows have shown the typical differentiation dynamics with formation of lobulo-alveolar structures in in vitro culture. This suggests the existence of bipotential mammary epithelial stem cells in the mammary gland. The strong mRNA expression of pluripotency factors indicates stemness, whereas expression of milk protein genes and epithelial cell-specific gene indicate their differentiation capabilities. Further, immunostaining results have shown the differentiation capabilities of KHMESCs into both luminal and basal lineages under the extracellular matrix (ECM, matrigel) free environment. However, under matrigel, the differentiation process was comparatively higher than without matrigel. Immunostaining results also suggested that differentiated cells could secrete milk proteins such as β-...Continue Reading

References

May 1, 1992·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A J AthertonB A Gusterson
Feb 1, 1992·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·N P PerusingheB A Gusterson
Oct 15, 1991·Biochemical and Biophysical Research Communications·R E Goodman, F L Schanbacher
Jan 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M L LiM J Bissell
Oct 30, 1971·Lancet·M Seabright
May 1, 1974·The Journal of Sports Medicine·D B SlocumS L James
Apr 29, 1999·Journal of Mammary Gland Biology and Neoplasia·R C HoveyR M Akers
Jul 30, 1999·Journal of Mammary Gland Biology and Neoplasia·V M Weaver, M J Bissell
Oct 9, 2002·The Journal of Dairy Research·Michael T RoseYoshiaki Obara
Feb 27, 2003·Chemico-biological Interactions·Nickolas A Sophos, Vasilis Vasiliou
Sep 8, 2004·Veterinary Immunology and Immunopathology·Olga Wellnitz, David E Kerr
Jan 6, 2006·Nature·John StinglConnie J Eaves
Jan 7, 2006·Nature·Mark ShackletonJane E Visvader
Jul 22, 2006·Proceedings of the National Academy of Sciences of the United States of America·John P ChuteDonald P McDonnell
Jan 26, 2007·The International Journal of Biochemistry & Cell Biology·Elad Katz, Charles H Streuli
May 23, 2007·Journal of Dairy Science·J Szyda, J Komisarek
Nov 22, 2007·Science·Junying YuJames A Thomson
Jun 25, 2008·Stem Cells and Development·Julie DouvilleDanuta Balicki
Dec 25, 2008·Functional & Integrative Genomics·Karensa K MenziesKevin R Nicholas
Jul 9, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Martine H A M van MiltenburgBob van de Water
Aug 13, 2010·Cold Spring Harbor Perspectives in Biology·John Muschler, Charles H Streuli
Oct 12, 2010·Cold Spring Harbor Perspectives in Biology·Jane E Visvader, Gilbert H Smith
Mar 29, 2011·Acta histochemica·Mohamed AlkafafyAmr Helal
Oct 11, 2011·Nature·Alexandra Van KeymeulenCédric Blanpain
Jan 19, 2012·PloS One·Gat Rauner, Itamar Barash
Jan 25, 2012·Gene Expression·Robert D Bruno, Gilbert H Smith
Jan 25, 2012·In Vitro Cellular & Developmental Biology. Animal·Hui-Li TongDe-Yun Yin
May 12, 2012·Stem Cells and Development·Jan H SpaasGerlinde R Van de Walle
Nov 28, 2012·In Vitro Cellular & Developmental Biology. Animal·Ramakant KaushikManmohan Singh Chauhan
Aug 29, 2013·International Journal of Biological Sciences·Neelesh Sharma, Dong Kee Jeong
Jan 1, 2013·Annual Review of Animal Biosciences·Anthony V Capuco, Steven E Ellis

❮ Previous
Next ❯

Citations

Sep 1, 2015·Experimental Cell Research·Eugenio MartignaniMario Baratta
Aug 28, 2021·Journal of Animal Science and Technology·Pavan KumarAwis Qurni Sazili

❮ Previous
Next ❯

Methods Mentioned

BETA
mechanical dissociation
transmission electron microscopy
ELISA
electrophoresis
PCR
Assay
flow cytometry

Software Mentioned

imageJ
primer

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.