PMID: 5918574Sep 2, 1966

Differentiation in vitro: effects of Sephadex fractions of chick embryo extract

H G Coon, R D Cahn


Chicken embryo extract has been fractionated into high-and low-molecular-weight components on Sephadex G-25. Media supplemented with the low-molecular-weight fraction (L) support full differentiation in clones of cartilage and of pigmented retina cells from chicken embryos. Growth rates of such cultures in L-supplemented media are greater than in media without embryo extract, and plating efficiencies are higher than in media with or without whole embryo extract. The high-molecular-weight fraction (H) in low concentrations also stimulates growth and plating efficiency, but inhibits the expression of differentiation.


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Related Concepts

Cell Differentiation Process
Chick Embryo
Tissue Extracts

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