PMID: 9182862Jan 1, 1997Paper

Differentiation induction of HL60 cells by 1,25(OH)2D3, all trans retinoic acid, rTGF-beta2 and their combinations

The Journal of Steroid Biochemistry and Molecular Biology
L VerlindenR Bouillon

Abstract

1,25 Dihydroxyvitamin D3 (D3), all trans retinoic acid (atRA) and the cytokine rTGF-beta2 are growth and differentiation modulators of promyelocytic leukemia. D3 gives rise to a functional monocytic cell population whereas single atRA therapy induces granulocytic cell features. rTGF-beta2 reduces HL60 cell proliferation but has no differentiating capacity. Combination treatment demonstrates additive effects between either D3 and atRA or D3 and rTGF-beta2, resulting in a cell population with mixed characteristics since individual cells exhibit both monocytic as granulocytic cell features. The capacity of single and combined treatments to induce a permanent differentiation was investigated. Therefore, cells were preincubated with the drugs during six days, test drugs were removed and cell number was monitored. The total cell count of populations treated with single agents remains constant for only a few days and then increases rapidly. rTGF-beta2 cooperated with D3 in inducing a long-lasting differentiation state (3 weeks). Addition of atRA to this combination did not significantly alter proliferation or differentiation, but some cells underwent apoptosis. Therefore, a total and permanent differentiation of leukemic cells may be ...Continue Reading

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Citations

Apr 20, 2007·Leukemia·M B Miranda, D E Johnson
Apr 27, 2011·Journal of Hematology & Oncology·Pan JianNi Jian
Feb 23, 2005·Immunity·Kee Nyung LeeInpyo Choi
Jan 11, 2007·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Christine M KitsosDouglas Levinson
May 7, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Miroslava KrzyzankovaRenata Veselska
Jul 22, 2004·Experimental Cell Research·Michael Danilenko, George P Studzinski

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