Abstract
Sebocyte differentiation is a continuous process, but its potential molecular mechanism remains unclear. We aimed to establish a novel sebocyte differentiation model using human primary sebocytes and to identify the expression profiles of differentiation-associated proteins. Primary human sebocytes were cultured on Sebomed medium supplemented with 2% serum for 7 days. Flow cytometry showed that S phase cells were decreased time-dependently, while G1 and subG1 (apoptosis) phase cells increased under serum starvation. Transmission electron microscopy and Oil Red O staining revealed a gradual increase of intracellular lipid accumulation. Expression of proliferation marker was diminished, while expression of differentiation, apoptosis, and lipogenic markers elevated gradually during 7-day culture. iTRAQ analysis identified 3582 expressed proteins in this differentiation model. Compared with day 0, number of differentially expressed proteins was 132, 54, 321, and 96 at days 1, 3, 5, and 7, respectively. Two overexpressed proteins (S100 calcium binding protein P and ferredoxin reductase) and 2 downexpressed proteins (adenosine deaminase and keratin 10) were further confirmed by Western blot and immunohistochemistry.
References
Jan 1, 1991·Skin Pharmacology : the Official Journal of the Skin Pharmacology Society·C C ZouboulisC E Orfanos
Aug 1, 1994·Experimental Dermatology·C C ZouboulisC E Orfanos
Jan 25, 2003·The Journal of Investigative Dermatology·Anna WróbelChristos C Zouboulis
Feb 1, 2005·European Journal of Cell Biology·Julia ReicheltThomas M Magin
Jan 18, 2006·Cell Cycle·Elisabetta SbisàApollonia Tullo
Oct 26, 2007·The Journal of Investigative Dermatology·Il HongMi-Ock Lee
Nov 3, 2007·Journal of Lipid Research·K R Smith, D M Thiboutot
Nov 9, 2007·The Journal of Investigative Dermatology·Terry M SmithDiane Thiboutot
Jan 19, 2008·Endocrinology·Yasutomi KameiYoshihiro Ogawa
May 14, 2008·Experimental Dermatology·Christos C ZouboulisAnja Thielitz
Dec 1, 2009·The International Journal of Biochemistry & Cell Biology·Marlon R Schneider, Ralf Paus
Feb 16, 2010·Journal der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG·Bodo C Melnik
Feb 18, 2011·Experimental Dermatology·Ichiro KurokawaRoland Moll
Mar 9, 2011·Pflügers Archiv : European journal of physiology·Balázs I TóthTamás Bíró
Apr 19, 2011·Proteomics·Yuexi WangRichard D Smith
Apr 14, 2012·Molecular & Cellular Proteomics : MCP·Annsofi SandbergJanne Lehtiö
Jan 3, 2013·The British Journal of Dermatology·H H KwonD H Suh
Apr 30, 2013·Journal of Dermatological Science·Li ZhuMasutaka Furue
May 22, 2013·Biochimica Et Biophysica Acta·Maik DahlhoffMarlon R Schneider
Jun 13, 2013·Molecular & Cellular Proteomics : MCP·Ruedi AebersoldRalph A Bradshaw
Jul 16, 2013·Journal of Dermatological Science·Zheng Jun LiMyung Im
Oct 12, 2013·PloS One·Anne-Sophie BorowiecGabriel Bidaux
Oct 17, 2013·The Journal of Investigative Dermatology·Aniko DozsaLaszlo Nagy
May 24, 2014·European Psychiatry : the Journal of the Association of European Psychiatrists·Y ZhanP Xie
Oct 23, 2014·Journal of Proteome Research·Tanveer S BatthJesper V Olsen
Sep 21, 2015·Journal of the American Academy of Dermatology·Vivian Y ShiRaja K Sivamani
Nov 27, 2015·Developmental Cell·Aparna BhaduriPaul A Khavari
Jan 6, 2016·International Journal of Molecular Sciences·Hyun-Kyoung YoonYoung-Choon Lee
Oct 12, 2016·Reviews in Endocrine & Metabolic Disorders·Christos C ZouboulisMarlon R Schneider
Oct 25, 2016·Journal of Dermatological Science·Ge ShiYi-Ming Fan
Oct 25, 2016·The Journal of Investigative Dermatology·Heinz FischerLeopold Eckhart
Apr 6, 2017·Cancer Research·Eun Mi KimHong-Duck Um