PMID: 7541758Jun 15, 1995Paper

Differentiation of Bartonella species using restriction endonuclease analysis of PCR-amplified 16S rRNA genes

FEMS Microbiology Letters
R J Birtles

Abstract

Differentiation of the four Bartonella species which were formerly classified as Rochalimaea using restriction endonuclease analysis of PCR-amplified citrate synthase gene fragments has previously been described. However, attempts to extend this method to include all members of Bartonella were confounded when amplification of the gene fragment from strains of B. bacilliformis each yielded two products of differing sizes. An alternative differentiation scheme for Bartonella species was developed based on restriction endonuclease analysis of their 16S rRNA genes. As the complete 16S rRNA gene sequences of all extant Bartonella species are available, the usefulness of specific endonucleases could be theoretically predetermined rather than discovered empirically. The potential usefulness of the restriction enzymes DdeI and MnlI was established using this approach, and this potential was confirmed in practice as all eight species could be distinguished from each other.

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Citations

Feb 7, 2001·Journal of Clinical Microbiology·P RenestoD Raoult
Mar 8, 2005·Journal of Clinical Microbiology·Ricardo G Maggi, Edward B Breitschwerdt
Jul 1, 1997·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·M MaurinD Raoult
Aug 6, 2004·Molecular and Cellular Probes·Alessandra Ciervo, Lorenzo Ciceroni
Sep 11, 2012·Journal of Microbiological Methods·Sarah N BussKimberlee A Musser
Jan 19, 2013·Revista Do Instituto De Medicina Tropical De São Paulo·Karina Hatamoto KawasatoThelma Suely Okay
Oct 11, 2013·Ocular Immunology and Inflammation·Ana Luiza Biancardi, Andre Luiz Land Curi
Mar 15, 2014·Revista Do Instituto De Medicina Tropical De São Paulo·Rodrigo StaggemeierVlademir Vicente Cantarelli
Mar 7, 2000·Clinical and Diagnostic Laboratory Immunology·D SwegerB Anderson
Jan 27, 2004·International Journal of Systematic and Evolutionary Microbiology·Renaud MaillardMuriel Vayssier-Taussat

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Bartonellosis is an infection caused by bacteria in the genus Bartonella and can present as a variety of diseases depending on the organism involved. While asymptomatic infection has been reported, immunocompromised individuals are more susceptible to severe infection. Find the latest research on bartonellosis here.