Abstract
Borrelia burgdorferi sensu lato is a multispecies complex of pathogenic spirochetes, causing Lyme borreliosis. Due to clinical, epidemiological, and taxonomical implications, there is a need for identification of isolated Borrelia strains. In the present study, we have optimized TGGE for B. burgdorferi sensu lato species differentiation and the results were compared with two reference methods, namely PFGE and restriction of 5S-23S intergenic space region PCR product. A differentiation of B. garinii, B. afzelii, and B. burgdorferi senso stricto species with TGGE was possible and intraspecies variation was detected. Results compared between TGGE, PFGE, and restriction of 5S-23S intergenic space region PCR product showed no difference in specificity of species identification.
References
Jan 1, 1992·Journal of Bacteriology·R T Marconi, C F Garon
Jul 1, 1992·International Journal of Systematic Bacteriology·J WelshM McClelland
May 9, 1987·Biophysical Chemistry·V Rosenbaum, D Riesner
Jan 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·V C SheffieldR M Myers
May 10, 1985·Nucleic Acids Research·R M MyersL S Lerman
Jun 18, 1982·Science·W BurgdorferJ P Davis
Dec 1, 1994·Electrophoresis·C GelfiM Ferrari
May 1, 1995·Research in Microbiology·T Balmelli, J C Piffaretti
May 1, 1995·Electrophoresis·U BuschV Preac-Mursic
Aug 1, 1993·FEMS Microbiology Letters·D RalphM McClelland
Oct 1, 1994·International Journal of Systematic Bacteriology·D PosticG Baranton
Nov 1, 1993·Journal of Clinical Microbiology·J BelfaizaI S Girons
Dec 24, 1997·Nature·C M FraserJ C Venter
May 1, 1998·Zentralblatt Für Bakteriologie : International Journal of Medical Microbiology·S O'ConnellG Stanek
Nov 25, 1998·Biophysical Journal·N KorolevL Nordenskiöld
Aug 17, 1999·Journal of Clinical Microbiology·D PosticG Baranton
Oct 9, 1999·Clinical Microbiology Reviews·G WangJ Dankert
Feb 1, 2000·Zentralblatt Für Bakteriologie : International Journal of Medical Microbiology·F Strle
Aug 31, 2000·Journal of Medical Microbiology·H J MonsteinJ Jonasson
Mar 15, 2001·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·P Parola, D Raoult
Apr 12, 2001·Letters in Applied Microbiology·A VásquezG Molin
Apr 24, 2001·Research in Microbiology·G BarantonD Dykhuizen
May 19, 2001·Electrophoresis·C T Hu, K M O'Shaughnessy
Jun 30, 2001·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·H J MonsteinJ Jonasson
Jul 23, 2002·Journal of Medical Microbiology·Angela ShinaCraig Winstanley
Jan 14, 2003·Gut·P SeksikJ Doré
Nov 25, 2003·Lancet·Gerold Stanek, Franc Strle
Jan 26, 2005·FEMS Microbiology Letters·Michie Yasuda, Michael Peter Shiaris