Differentiation of Borrelia burgdorferi sensu lato species by temperature gradient gel electrophoresis

Electrophoresis
Janez SimencGorazd Avgustin

Abstract

Borrelia burgdorferi sensu lato is a multispecies complex of pathogenic spirochetes, causing Lyme borreliosis. Due to clinical, epidemiological, and taxonomical implications, there is a need for identification of isolated Borrelia strains. In the present study, we have optimized TGGE for B. burgdorferi sensu lato species differentiation and the results were compared with two reference methods, namely PFGE and restriction of 5S-23S intergenic space region PCR product. A differentiation of B. garinii, B. afzelii, and B. burgdorferi senso stricto species with TGGE was possible and intraspecies variation was detected. Results compared between TGGE, PFGE, and restriction of 5S-23S intergenic space region PCR product showed no difference in specificity of species identification.

References

Jan 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·V C SheffieldR M Myers
Jun 18, 1982·Science·W BurgdorferJ P Davis
Aug 1, 1993·FEMS Microbiology Letters·D RalphM McClelland
Nov 1, 1993·Journal of Clinical Microbiology·J BelfaizaI S Girons
May 1, 1998·Zentralblatt Für Bakteriologie : International Journal of Medical Microbiology·S O'ConnellG Stanek
Aug 17, 1999·Journal of Clinical Microbiology·D PosticG Baranton
Feb 1, 2000·Zentralblatt Für Bakteriologie : International Journal of Medical Microbiology·F Strle
Mar 15, 2001·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·P Parola, D Raoult
Jun 30, 2001·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·H J MonsteinJ Jonasson
Nov 25, 2003·Lancet·Gerold Stanek, Franc Strle

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