PMID: 16523702Mar 10, 2006Paper

Differentiation of capripoxvirus species and strains by polymerase chain reaction

Molekuliarnaia biologiia
E S OrlovaV M Zakharov

Abstract

A fast and simple method for capripoxvirus species identification has been developed. The method is based on multiplex polymerase chain reaction (MPCR) with species-specific primers and does not require nucleotide sequencing or restriction analysis of PCR products. To differentiate vaccine stains used in Russia and countries of the former Soviet Union from epizootic isolates of sheep pox virus, a method based on restriction analysis of the ankyrin-repeat protein gene fragment amplified by PCR has been developed. Being highly specific, both methods may be used for routine diagnosis of capripoxvirus-associated diseases.

References

May 1, 1985·Tropical Animal Health and Production·R P Kitching, W P Taylor
Apr 1, 1989·Epidemiology and Infection·R P KitchingD N Black
Aug 1, 1986·Virus Research·D N BlackR P Kitching
Oct 8, 1998·Journal of Virological Methods·D C Ireland, Y S Binepal
Feb 24, 1999·Journal of Virological Methods·O Mangana-VougioukaO Papadopoulos
May 22, 2002·Journal of Virology·E R TulmanD L Rock

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Citations

Jan 12, 2011·Virologica Sinica·A A El-Kenawy, M S El-Tholoth
Sep 9, 2008·Transboundary and Emerging Diseases·S BabiukR P Kitching
Jul 14, 2011·Transboundary and Emerging Diseases·E S M Tuppurainen, C A L Oura
Oct 30, 2010·Journal of Virological Methods·Charles Euloge LamienAdama Diallo
Dec 6, 2011·Journal of Virological Methods·Samuel StubbsEeva S M Tuppurainen
Jul 16, 2013·Journal of Virological Methods·A HaegemanK De Clercq
Jun 29, 2017·Transboundary and Emerging Diseases·F Ben ChehidaC Cêtre-Sossah
Aug 29, 2020·Veterinary Research Communications·Tania GuptaRajesh Chahota

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