PMID: 8604543Nov 1, 1995Paper

Differentiation of classical swine fever virus (CSFV) strains using monoclonal antibodies against structural glycoproteins

Veterinary Microbiology
A KosmidouE Weiland

Abstract

Two panels of monoclonal antibodies (mAbs) against the classical swine fever virus (CSFV) envelope glycoproteins E2 (12 mAbs) and E0 (11 mAbs) were established and tested by immunoperoxidase binding assay against 135 pestivirus strains and isolates. Variability of the binding pattern was demonstrated for CSFV and also for bovine viral diarrhea virus (BVDV) strains and isolates. The panels of mAbs against E2 and E0 led to very different reactivity patterns. Particular mAbs against E2 reacted with (i) all CSFV isolates, (ii) only 4 out of 126 CSFV isolates, or (iii) about 90% of the tested CSFV isolates and 78% of ruminant pestivirus isolates. Anti CSFV E0 mAbs allowed the detection of a greater variability among the CSFV strains and isolates than the anti E2 mAbs. None of the 11 anti E0 mAbs recognized an epitope conserved for CSFV or showed crossreactivity with ruminant pestiviruses. The use of both panels of mAbs against two CSFV structural glycoproteins led to the discrimination of 21 antigenic types of CSFV strains and isolates. The described panels can be used to trace the origin of CSFV after outbreaks of the disease.

References

Jan 1, 1990·Archives of Virology·I Greiser-WilkeB Liess

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Citations

Dec 2, 2008·Tropical Animal Health and Production·James V ConlanLaurence J Gleeson
Dec 31, 1998·Journal of Virological Methods·I Greiser-WilkeV Moennig
Apr 28, 2000·Veterinary Microbiology·D J PatonS Belák
Mar 6, 2003·The Veterinary Journal·V MoennigI Greiser-Wilke
Jul 1, 1997·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·L Haas
Aug 29, 2006·Vaccine·Xiao-Nan Dong, Ying-Hua Chen
Feb 14, 2002·The Journal of General Virology·Elisenda ArmengolArmin Saalmüller
Jun 5, 2004·Biochemical and Biophysical Research Communications·Norica Branza-NichitaNicole Zitzmann
Nov 7, 2006·Veterinary Dermatology·David McEwan JenkinsonDouglas L Bovell
Aug 25, 2012·Uirusu·Yoshihiro Sakoda
Jul 25, 2021·International Journal of Molecular Sciences·Yu MuGregor Meyers

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