Differentiation of early germ cells from human skin-derived stem cells without exogenous gene integration

Scientific Reports
Wei GeWei Shen

Abstract

Infertility has long been a difficult issue for many couples. The successful differentiation of germ cells and live progeny from pluripotent stem cells brings new hope to the couples suffering with infertility. Here we successfully isolated human fetus skin-derived stem cells (hfSDSCs) from fetus skin tissue and demonstrated that hfSDSCs can be differentiated into early human germ cell-like cells (hGCLCs). These cells express human germ cell markers DAZL and VASA. Moreover, these pluripotent stem cell-derived hGCLCs are free of exogenous gene integration. When hfSDSCs were differentiated in porcine follicle fluid (PFF) conditioned media, which has been shown to promote the differentiation of mouse and porcine SDSCs into oocyte-like cells (OLCs), we observed some vesicular structures formed from hfSDSCs. Moreover, when hfSDSCs were cultured with specific conditioned media, we observed punctate and elongated SCP3 staining foci, indicating the initiation of meiosis. Ploidy analysis and fluorescent in situ hybridization (FISH) analysis indicated that a small percentage of putative 1N populations formed from hfSDSCs when compared with positive controls. In conclusion, our data here, for the first time, demonstrated that hfSDSCs poss...Continue Reading

References

Sep 25, 2003·Proceedings of the National Academy of Sciences of the United States of America·Yayoi ToyookaToshiaki Noce
Feb 14, 2004·Human Molecular Genetics·Amander T ClarkRenee A Reijo Pera
Mar 23, 2004·Biochemical and Biophysical Research Communications·Paul W DyceJulang Li
Jan 18, 2006·Journal of Investigative Medicine : the Official Publication of the American Federation for Clinical Research·Nigel M de S Cameron
Mar 28, 2006·Nature Cell Biology·Paul W DyceJulang Li
Jan 27, 2007·Stem Cells and Development·Kehkooi KeeRenee A Reijo Pera
Nov 9, 2010·Stem Cells and Development·Paul W DyceJulang Li
Dec 7, 2010·Human Molecular Genetics·Sarita PanulaRenee A Reijo Pera
May 17, 2011·Nature·Tongbiao ZhaoYang Xu
Jun 18, 2011·Stem Cells·C EguizabalJ C Izpisua Belmonte
Aug 7, 2012·Cell & Bioscience·Lu WangLixin Feng
Aug 28, 2012·Cell Reports·Charles A EasleyGerald P Schatten
Feb 2, 2013·Circulation Research·Hideyuki OkanoKyoko Miura
Jan 2, 2014·Development·Erna Magnúsdóttir, M Azim Surani
Jan 23, 2014·Human Molecular Genetics·Jens Durruthy DurruthyRenee A Reijo Pera
Jun 7, 2014·Cell·Mary Ann HandelJohn C Schimenti

❮ Previous
Next ❯

Citations

Oct 30, 2016·Acta Biochimica Et Biophysica Sinica·Dongmei LaiCharlie Xiang
Nov 18, 2017·Reproductive Biology and Endocrinology : RB&E·Deepa BhartiyaSeema Parte
Mar 2, 2018·Journal of Ovarian Research·Paul W DyceGerald M Kidder
May 6, 2020·Cellular and Molecular Life Sciences : CMLS·Wei GeWei Shen
Oct 28, 2019·Journal of Cellular Biochemistry·Javad Amini MahabadiHossein Nikzad
Dec 28, 2017·Human Reproduction Update·João Pedro Alves-Lopes, Jan-Bernd Stukenborg

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
PCR

Software Mentioned

Image Pro Plus
CellQuest
GraphPad Prism

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.