Differentiation of ejaculates showing reactive oxygen species production by spermatozoa or leukocytes

Andrologia
Ralf HenkelW B Schill

Abstract

Differences between subgroups and correlations between reactive oxygen species (ROS), sperm motility, concentration of leukocytes and viability in semen samples from 143 men were investigated. Patients with azoospermia or leukocytospermia were excluded from the study. Spermatozoa were separated by means of glass wool filtration. Reactive oxygen species were determined by means of luminol chemiluminescence before and after sperm separation; thereafter, normozoospermic and oligozoospermic patients were divided into three subgroups using the mean of all patients investigated (17462 count 10(-7) viable spermatozoa) as cut-off value as follows: G1--high reactive oxygen species production in native semen and after glass wool filtration; G2--high production of reactive oxygen species only in native semen; G3--low levels of reactive oxygen species before and after glass wool filtration. In general, reactive oxygen species were significantly higher in oligozoospermic samples than in normozoospermic samples. In men with normal sperm count, ROS production correlated significantly with the number of leukocytes in the ejaculate before glass wool filtration, but not thereafter. Glass wool filtration is useful to distinguish between sperm sam...Continue Reading

References

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Citations

Mar 9, 1999·International Journal of Andrology·T IchikawaW B Schill
Mar 7, 2005·Reproductive Medicine and Biology·Ralf HenkelWolf-Bernhard Schill
Jul 18, 2002·International Journal of Andrology·R John Aitken, Mark A Baker
May 30, 2002·Biological Chemistry·Matilde Maiorino, Fulvio Ursini
Jun 19, 2012·Reproductive Biology and Endocrinology : RB&E·Mario CavagnaJosé G Franco
Jun 18, 1998·Andrologia·R Henkel, W B Schill
May 1, 2012·Reproduction, Fertility, and Development·Mohammad Bozlur RahmanAnn Van Soom
Jul 10, 2001·Archives of Andrology·J M Nani, R S Jeyendran

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