Abstract
Hypothalmic tissue from 16 to 18-day fetal rats was transplanted onto the choridal pia overlying the superior colliculus in adult female rats. After survival periods of 2 weeks to 19 months, brains containing transplants were processed for monoamine fluorescence histochemistry, immunohistochemistry for three neuropeptides (LHRH, somatostatin, neurophysin), or for autoradiography in ovariectomized hosts that received [3 H] estradiol. Most of the transplants survived and retained or increased in size; 14 of 25 transplants examined by fluorescence histochemistry were found to contain median eminence-like structures. In almost all of the transplants that were stained for neuropeptides, beaded processes and occasional cell bodies were observed. Although immunoreactive fibers were found near blood vessels, no palisade arrangement typical of the normal median eminence was evident. Each of the hypothalamic transplants on which steroid autoradiography was performed contained clusters of estrophilic neurons, the intensity of labeling of which was comparable to that seen in the host hypothalamus. These results indicate that many characteristic morphological and chemical features of the hypothalamus, which are not evident in the 16 to 18-d...Continue Reading
Citations
Jan 1, 1990·Developmental Biology·S DaikokuH Daikoku-Ishido
Nov 1, 1983·Brain Research·P J ReierL Guth
Dec 1, 1983·Brain Research·W J FriedmanJ L Gerlach
May 1, 1987·Brain Research·C M Paden, C E Roselli
Jan 1, 1984·Peptides·D E ScottD M Gash
Jan 1, 1985·Neurobiology of Aging·D M GashJ R Sladek
Oct 24, 1985·Neuroscience Letters·D E Scott
Aug 1, 1985·Neuroscience·G J BoerN Schluter
Jun 1, 1987·Neuroscience·R M LindsayP J Seeley
Jan 1, 1988·Brain Research Bulletin·S J Wiegand, D M Gash
Nov 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·M Nishizuka, Y Arai
Jan 1, 1988·Journal of Neurosurgery·R K EricksonR L Wollmann
Aug 1, 1989·Experimental Neurology·M J GibsonJ I Morrell
Jan 1, 1982·Annals of the New York Academy of Sciences·J R SladekD M Gash
Mar 1, 1982·Journal of Neurochemistry·R H SchmidtA Björklund
Feb 19, 1986·Brain Research·A W DeckelR G Robinson
Aug 20, 1983·The Journal of Comparative Neurology·L F KromerU Stenevi
Jul 29, 1982·Nature·D T KriegerH M Charlton
Jan 1, 1985·The Journal of Comparative Neurology·C B Jaeger
Jan 1, 1985·Diabetes/metabolism Reviews·B JeanrenaudG van de Werve
Jun 1, 1980·Molecular and Cellular Endocrinology·B S McEwen
Jan 1, 1991·Experimental Neurology·L C DoeringA J Aguayo