Differentiation of equine induced pluripotent stem cells into a keratinocyte lineage

Equine Veterinary Journal
C AguiarC Theoret

Abstract

Skin trauma in horses often leads to the development of chronic nonhealing wounds that lack a keratinocyte cover, vital for healing. Reports in mouse and man confirm the possibility of generating functional keratinocytes from induced pluripotent stem cells (iPSC), thus presenting myriad potential applications for wound management or treatment of skin disease. Similarly, differentiation of equine iPSC (eiPSC) into a keratinocyte lineage should provide opportunities for the advancement of veterinary regenerative medicine. The purpose of this study was to develop an efficient method for the differentiation of eiPSC into a keratinocyte lineage. It was hypothesised that eiPSC can form differentiated keratinocytes (eiPSC-KC) comparable with primary equine keratinocytes (PEK) in their morphological and functional characteristics. Experimental in vitro study. Equine iPSC established using a nonviral system were treated for 30 days with retinoic acid and bone morphogenetic protein-4 to induce directed differentiation into iPSC-KC. Temporospatial gene and protein expression by eiPSC-KC was measured at weekly intervals of differentiation and in response to calcium switch. Proliferative and migratory capacities of eiPSC-KC were compared wi...Continue Reading

References

Dec 1, 1990·The Journal of Cell Biology·E Fuchs
Mar 21, 2002·Equine Veterinary Journal·A M DahmM M Suter
Jan 10, 2003·The Journal of Investigative Dermatology. Symposium Proceedings·Maranke I KosterDennis R Roop
Sep 17, 2004·The Journal of Investigative Dermatology. Symposium Proceedings·Pritinder KaurIvan Bertoncello
Oct 17, 2008·Molecular Biology of the Cell·Timo LiebigVania M M Braga
Feb 11, 2009·The Veterinary Clinics of North America. Equine Practice·R Reid Hanson
Dec 19, 2009·Veterinary Surgery : VS·Christine Theoret
Dec 15, 2010·The Journal of Investigative Dermatology·Ganna BilousovaDennis R Roop
Feb 25, 2011·Stem Cell Reviews and Reports·Kristina NagyAndras Nagy
May 17, 2011·Nature·Tongbiao ZhaoYang Xu
Sep 17, 2011·Circulation Research·Keisuke OkitaShinya Yamanaka
May 3, 2012·Stem Cells International·Khodadad KhodadadiPaul J Verma
Aug 31, 2012·The Journal of Investigative Dermatology·Jakub TolarJohn E Wagner
Dec 14, 2012·The Journal of Investigative Dermatology·Fahad K KidwaiTong Cao
Sep 10, 2014·Equine Veterinary Journal·C AguiarL C Smith

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Citations

Oct 30, 2015·International Journal of Molecular Sciences·Nkemcho OjehOlivera Stojadinovic
Dec 15, 2015·Frontiers in Veterinary Science·F Xavier Donadeu, Cristina L Esteves
Apr 14, 2017·BioTechniques·João Paulo Silva Nunes, Adriana Abalen Martins Dias
Jul 6, 2017·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Y Z PatersonD J Guest
Oct 25, 2017·Stem Cells Translational Medicine·Jamie A TextorDori L Borjesson
Jul 31, 2019·Stem Cells and Development·Xiaoqiang CongJiesi Luo
Sep 17, 2019·World Journal of Stem Cells·Laís Vicari de Figueiredo PessôaKristine Karla Freude
Oct 25, 2020·Journal of Animal Science·Yue SuYoung Tang
Dec 10, 2020·BMC Veterinary Research·Rachel A ScarfoneThomas G Koch
Feb 17, 2021·Veterinary Research·Laurent Souci, Caroline Denesvre
May 11, 2021·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Subholakshmi ChoudhuryAmitava Das
May 28, 2021·Advances in Wound Care·Rebecca M HarmanGerlinde R Van de Walle

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