Differentiation of eye field neuroectoderm from human adipose-derived stem cells by using small-molecules and hADSC-conditioned medium

Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft
Noushin AmirpourHossein Salehi

Abstract

Recently, stem-cell therapy as a promising therapeutic alternative is considered to treat retinal degenerative diseases. Here, we used small molecules and concentrated conditioned medium selectively enriched with Amicon filter units from human adipose-derived stem cells (hADSC-CM) containing various neurotrophic factors to induce hADSCs toward eye field neuroectoderm (EFN). For induction of stem cells, hADSC-CM and small molecules CKI-7, SB431542 and LDN193189 as inhibitors of Wnt, Nodal and BMP4 signaling pathways were used, respectively. We found the highest expression of β-TUB III as a neural marker in the group in which small molecules and conditioned medium were applied simultaneously. Moreover, EFN markers SIX3, PAX6 and RAX had higher expression in the presence of a conditioned medium. However, the superior expression of ENF marker OTX2 was seen in the small molecules group. Our results indicated that neurotrophic factors present in hADSCs-CM could induce hADSCs into EFN cells. Therefore, a more thorough study of these factors and their effects in hADSC-CM might pave the way for cellular and non-cellular therapy in retinal degenerative diseases.

Citations

Apr 24, 2020·Journal of Cellular Physiology·Mehnoosh Ashja-ArvanMazdak Ganjalikhani-Hakemi
Jan 8, 2021·Stem Cell Reviews and Reports·Sanjucta AdakBithiah Grace Jaganathan
Mar 7, 2021·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·Bei-Yong ShenQi Zhou
Jun 30, 2021·Molecular Biology Reports·Samira Shariati NajafabadiHossein Salehi

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