PMID: 6167989Jun 1, 1981Paper

Differentiation of fetal liver cells in vitro

Proceedings of the National Academy of Sciences of the United States of America
A E FreemanE Ruoslahti

Abstract

Fetal mouse liver hepatocytes proliferate on a substrate of irradiated pigskin epidermis scored with scalpel blade slits to permit cell access to the basement membrane. At the time the cells are explanted, fetal genes, such as those responsible for production of alpha-fetoprotein (AFP) and gamma-glutamyltransferase (GGTase), are strongly expressed. The levels of GGTase decrease rapidly and become undetectable within 2 weeks. The levels of AFP decrease more gradually but become undetectable after 3-5 weeks in culture. As the AFP levels decrease, there is a concomitant increase in albumin production. Hydrocortisone prolongs production of AFP (for up to 8 weeks) but not of GGTase, and it decreases albumin production for up to 8 weeks. Once cells lose AFP expression, addition of hydrocortisone does not restart it. Based on these data, fetal mouse liver hepatocytes, cultured on pigskin, seem to be an excellent in vitro model for liver cell maturation.

References

Aug 21, 1975·Nature·L BelangerP M Gagnon
Jan 12, 1976·Biochemical and Biophysical Research Communications·B de NechaudV R Potter
Apr 29, 1976·Nature·E Ruoslahti, W D Terry
Apr 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·H LeffertI Arias
Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·A E SiricaH C Pitot
Aug 28, 1979·Biochemical and Biophysical Research Communications·P CommerJ F Chiu
Sep 1, 1977·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·L A Sternberger, J P Petrali
Sep 15, 1971·International Journal of Cancer. Journal International Du Cancer·M KekomäkiK Raivio
Mar 1, 1972·The Journal of Cell Biology·H L Leffert, D Paul
Nov 1, 1971·Experimental Cell Research·G M WilliamsJ H Weisburger
Apr 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·R CarlssonE Ruoslahti

❮ Previous
Next ❯

Citations

Dec 1, 1987·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·T TokiwaJ Sato
Sep 1, 1987·Cell Differentiation·A BollinneJ E Gielen
Mar 1, 1982·Collagen and Related Research·M Rojkind, P Ponce-Noyola
Jun 1, 1983·The Journal of Cell Biology·A GroverE D Adamson
Jul 1, 1983·The Journal of Cell Biology·A GroverE D Adamson
Sep 1, 1983·The Journal of Cell Biology·H Baumann, G P Jahreis
Jun 1, 1988·Archives of Biochemistry and Biophysics·J Y Chou
Mar 16, 1983·Biochemical and Biophysical Research Communications·T HattoriF Hirata
Jan 1, 1984·Differentiation; Research in Biological Diversity·E D Adamson, B L Hogan
Jan 1, 1985·Differentiation; Research in Biological Diversity·E D AdamsonB Kahan
Jan 1, 1986·Differentiation; Research in Biological Diversity·A VenetianerJ M Sala-Trepat
Nov 1, 1982·The Anatomical Record·J E KlaunigB F Trump

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.