PMID: 19122405Jan 6, 2009Paper

Differentiation of foot-and-mouth disease virus-infected pigs from vaccinated pigs using a western blotting assay based on baculovirus-expressed nonstructural proteins 2C and 3D

The Journal of Veterinary Medical Science
Katsuhiko FukaiKenichi Sakamoto

Abstract

Baculovirus-expressed foot-and-mouth disease virus (FMDV) nonstructural proteins 2C and 3D were used as the antigens in a western blotting assay. This assay allowed for differentiation of FMDV-infected pigs from vaccinated pigs. Serial studies were performed using sera collected from experimentally infected and vaccinated pigs. Positive reactions were found in the sera derived from the experimentally infected pigs. There was, however, no positive reaction in the vaccinated pigs. These results suggest that this western blotting assay is a useful method for differentiation of infected pigs from vaccinated pigs.

References

Apr 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·T E DeverW C Merrick
Jan 18, 1994·Proceedings of the National Academy of Sciences of the United States of America·J F NewmanF Brown
Feb 21, 2002·The Journal of Veterinary Medical Science·Kenichi SakamotoYosuke Murakami
Jan 16, 2003·Virus Research·G R ThomsonA D S Bastos

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Citations

Jul 16, 2013·Journal of Virological Methods·Sonalika MahajanBramhadev Pattnaik
Aug 19, 2009·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·David J PatonBryan Charleston

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