Differentiation of function among the RsbR paralogs in the general stress response of Bacillus subtilis with regard to light perception

Journal of Bacteriology
Jeroen B van der SteenKlaas J Hellingwerf

Abstract

The general stress response of Bacillus subtilis can be activated by a wide range of signals, including low intensities of visible light. It is regulated by a dedicated σ factor via a complex signal transduction pathway that makes use of stressosomes: hetero-oligomeric complexes that include one or more of the RsbR proteins (RsbRA, RsbRB, RsbRC, and RsbRD). The response to blue light is mediated by the photoreceptor YtvA. We show here which of the four RsbR proteins are necessary for the activation of the σ(B) response by blue light. Experiments performed with single-, double-, and triple-deletion strains in the rsbR genes show that RsbRB and RsbRA function antagonistically, with the former being a negative regulator and the latter a positive regulator of the YtvA-dependent light activation of the stress response. A strain with RsbRB as the only RsbR protein is unable to respond to light-activation of σ(B). Furthermore, RsbRC and RsbRD can replace RsbRA's function only in the absence of RsbRB. This differentiation of function is confined to light stress, since strains with RsbRA or RsbRB as the only RsbR protein behave similarly in our experimental conditions in response to physicochemical stresses. Interestingly, RsbRB's absen...Continue Reading

References

Dec 1, 1993·Journal of Bacteriology·S A BoylanChester W Price
Apr 1, 1993·Journal of Bacteriology·A K Benson, William Haldenwang
Sep 5, 2001·Molecular Microbiology·Chester W PriceP Youngman
Sep 7, 2001·Journal of Bacteriology·A PetersohnMichael Hecker
Nov 22, 2001·Journal of Bacteriology·J D HelmannC Paddon
Apr 20, 2002·Biophysical Journal·Aba LosiWolfgang Gärtner
Mar 29, 2003·Journal of Molecular Biology·Patrick EichenbergerRichard Losick
Sep 3, 2003·Molecular Microbiology·Chien-Cheng ChenOlivier Delumeau
Jun 2, 2004·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Aba Losi
Jul 21, 2004·The Journal of Biological Chemistry·Olivier DelumeauRichard J Lewis
Aug 18, 2004·Journal of Molecular Biology·Tae-Jong KimChester W Price
Dec 2, 2005·Journal of Molecular Microbiology and Biotechnology·Jan Pané-FarréJörg Stülke
Dec 13, 2005·Microbiology and Molecular Biology Reviews : MMBR·Mark J KazmierczakKathryn J Boor
Aug 23, 2006·Journal of Bacteriology·Tatiana A GaidenkoChester W Price
Aug 23, 2006·Journal of Bacteriology·Marcela Avila-PérezRemco Kort
Jun 19, 2007·The Journal of General and Applied Microbiology·Noriko SuzukiAkira Nakamura
Nov 24, 2007·Annual Review of Microbiology·Michael HeckerUwe Völker
Jul 8, 2009·The Journal of Biological Chemistry·Marcela Avila-PérezKlaas J Hellingwerf
Aug 26, 2009·Communicative & Integrative Biology·Jon Marles-Wright, Richard J Lewis

Citations

Jul 3, 2013·Molecular BioSystems·Jeroen B van der SteenKlaas J Hellingwerf
Feb 19, 2013·Biochemical and Biophysical Research Communications·Marcel JurkPeter Schmieder
Jul 21, 2015·Photochemistry and Photobiology·Jeroen B van der Steen, Klaas J Hellingwerf

Related Concepts

RsbR protein, Bacillus subtilis
Natto Bacteria
Bacterial Proteins
Phosphoproteins
Gene Expression Regulation, Bacterial
Light Signal Transduction
Bacillus subtilis
Cell Differentiation Process
Environment
Gene Deletion

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