Differentiation of Fusarium from Aspergillus species by colorimetric in situ hybridization in formalin-fixed, paraffin-embedded tissue sections using dual fluorogenic-labeled LNA probes

American Journal of Clinical Pathology
Kathleen T Montone

Abstract

Fusarium and Aspergillus are 2 genera of fungal pathogens that can result in devastating disease particularly in immunosuppressed hosts. In tissue sections, these organisms can be extremely difficult to distinguish from one another. To differentiate between these 2 pathogens, a rapid (<3 hours) ribosomal RNA (rRNA) in situ hybridization (ISH) protocol using dual fluorogenic-labeled oligonucleotide probes composed of a mixture of DNA and locked nucleic acids (LNAs) was developed. This assay was able to differentiate between Aspergillus and Fusarium in formalin-fixed, paraffin-embedded tissue sections. ISH targeting rRNA can be used to identify the species of fungal pathogens in surgical pathology material and may be useful when pathogens are histologically observed but cultures are negative or have not been performed. ISH with dual-labeled LNA probes may be useful for detecting a variety of fungal pathogens in formalin-fixed, paraffin-embedded tissue specimens.

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Citations

Nov 19, 2014·Future Microbiology·Carina AlmeidaNuno F Azevedo
Apr 13, 2011·Clinical Microbiology Reviews·Jeannette Guarner, Mary E Brandt
Sep 29, 2011·Current Infectious Disease Reports·Cornelia Mrazek, Cornelia Lass-Flörl
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Aug 25, 2015·Current Fungal Infection Reports·Anne D van DiepeningenCees Waalwijk
Feb 20, 2014·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·A M TortoranoUNKNOWN European Confederation of Medical Mycology
Jan 14, 2011·American Journal of Clinical Pathology·Kathleen T MontoneIrving Nachamkin
Nov 22, 2012·The American Journal of Dermatopathology·Ossama Abbas, Jag Bhawan
Feb 7, 2018·Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology·Prajwal BodduSteven Kornblau

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