PMID: 11918689Mar 29, 2002Paper

Differentiation of human alloreactive CD8(+) T cells in vitro

Immunology
Rob J RentenaarIneke J M ten Berge

Abstract

Expansion and differentiation of alloantigen-reactive CD8(+) T cells in mixed lymphocyte cultures was followed by measurement of the loss of carboxyfluorescein diacetate succinimidyl ester (CFSE) fluorescence of responder cells. Proliferation of CD8(+) T cells became detectable on day 4 of culture and, 2 days later, > 60% of the CD8(+) T cells in culture were dividing alloreactive lymphocytes. In parallel with expansion, CD8(+) T-cell differentiation was initiated, as evidenced by an increase in the number of CD45RA(-) and CD27(-) T cells and acquisition of the ability to produce interferon-gamma after restimulation with the specific alloantigen. Finally, although short-term stimulation and measurement of intracellular cytokine production allowed visualization of alloreactive CD8(+) T cells expanded in vitro, this procedure did not detect circulating alloreactive CD8(+) T cells activated in vivo in recipients of allogeneic kidney grafts.

References

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Jan 5, 1999·Scandinavian Journal of Immunology·R M O'Brien, D M Kemeny
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Mar 27, 2001·Transplantation Proceedings·R J RentenaarI J ten Berge

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Citations

Mar 31, 2009·Cancer Immunology, Immunotherapy : CII·Alban GervaisJacky Bernard
Mar 2, 2013·American Journal of Respiratory Cell and Molecular Biology·Naoko Kumagai-TakeiTakemi Otsuki
Oct 10, 2020·Environmental Health and Preventive Medicine·Naoko Kumagai-TakeiTakemi Otsuki

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