Differentiation of Human Dental Pulp Stem Cells into Dopaminergic Neuron-like Cells in Vitro

Journal of Korean Medical Science
So Young ChunEun Sang Yoo

Abstract

We investigated the potential of human dental pulp stem cells (hDPSCs) to differentiate into dopaminergic neurons in vitro as an autologous stem cell source for Parkinson's disease treatment. The hDPSCs were expanded in knockout-embryonic stem cell (KO-ES) medium containing leukemia inhibitory factor (LIF) on gelatin-coated plates for 3-4 days. Then, the medium was replaced with KO-ES medium without LIF to allow the formation of the neurosphere for 4 days. The neurosphere was transferred into ITS medium, containing ITS (human insulin-transferrin-sodium) and fibronectin, to select for Nestin-positive cells for 6-8 days. The cells were then cultured in N-2 medium containing basic fibroblast growth factor (FGF), FGF-8b, sonic hedgehog-N, and ascorbic acid on poly-l-ornithine/fibronectin-coated plates to expand the Nestin-positive cells for up to 2 weeks. Finally, the cells were transferred into N-2/ascorbic acid medium to allow for their differentiation into dopaminergic neurons for 10-15 days. The differentiation stages were confirmed by morphological, immunocytochemical, flow cytometric, real-time PCR, and ELISA analyses. The expressions of mesenchymal stem cell markers were observed at the early stages. The expressions of early...Continue Reading

References

Aug 1, 2000·Experimental Neurology·J Sanchez-RamosP R Sanberg
Aug 10, 2000·Journal of Neuroscience Research·D WoodburyI B Black
Nov 23, 2000·Proceedings of the National Academy of Sciences of the United States of America·S GronthosS Shi
Mar 28, 2003·Human Reproduction·Guido de Wert, Christine Mummery
Apr 30, 2003·Proceedings of the National Academy of Sciences of the United States of America·Masako MiuraSongtao Shi
Jul 23, 2003·CNS Drugs·Silvia MandelMoussa B H Youdim
Sep 16, 2003·Neuron·William Dauer, Serge Przedborski
Dec 4, 2003·Brain Research·Alessandra BezEugenio Agostino Parati
Feb 16, 2005·Developmental Neuroscience·Corinna Klein, Gord Fishell
Nov 17, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Sylvian Bauer, Paul H Patterson
Jul 28, 2007·Stem Cells·Katarzyna A TrzaskaPranela Rameshwar
Nov 28, 2007·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Yi WangWei-dong Le
Aug 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Rutger-Jan SwijnenburgJoseph C Wu
Jul 7, 2012·PloS One·Nelson F LizierIrina Kerkis
Dec 4, 2012·The Journal of Clinical Investigation·Takuya HayashiMari Dezawa

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Citations

Jan 25, 2017·ACS Applied Materials & Interfaces·Hatef Ghasemi HamidabadiMazaher Gholipourmalekabadi
Apr 4, 2017·European Journal of Clinical Investigation·Nareshwaran GnanasegaranNoor Hayaty Abu Kasim
Jul 8, 2017·IUBMB Life·Nareshwaran GnanasegaranNoor Hayaty Abu Kasim
Jul 25, 2017·Journal of Neuroscience Research·Ibrahim MortadaMohamad Al Bazzal
Oct 20, 2018·Journal of Biomedical Materials Research. Part a·Milda AlksneVirginija Bukelskiene
Apr 2, 2019·Critical Reviews in Clinical Laboratory Sciences·Aroa Relaño-GinésChristophe Hirtz
Jun 2, 2018·Stem Cells International·Lihua LuoQingsong Ye
Feb 1, 2019·International Journal of Molecular Sciences·Christopher SimonSivachandran Parimannan
Jul 30, 2020·Cell and Tissue Research·Duaa AbuarqoubAbdalla Awidi
Sep 20, 2017·Journal of Cellular Physiology·Debora Lo FurnoRosario Giuffrida
Sep 28, 2018·Stem Cell Research & Therapy·Syed Shadab RazaMohsin Ali Khan
Jan 19, 2019·International Journal of Molecular Sciences·Stefano MartellucciVincenzo Mattei
Nov 28, 2019·Stem Cells International·Rohaina Che ManMuhammad Dain Yazid
Jan 8, 2021·Stem Cell Research & Therapy·Zhuangzhuang XiaoHongwu Du
Mar 7, 2021·International Journal of Molecular Sciences·Keiji MasudaSatoshi Fukumoto
Apr 4, 2021·International Journal of Molecular Sciences·Jon LuzuriagaGaskon Ibarretxe
Apr 9, 2021·Stem Cell Reviews and Reports·Vincenzo MatteiSimona Delle Monache
Apr 20, 2021·Frontiers in Cell and Developmental Biology·Guoyang ZhouLin Wang
Aug 24, 2021·Research in Veterinary Science·Paulo Henrique UtumiJosé Ademar Villanova Junior

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Methods Mentioned

BETA
flow cytometry
fluorescence-activated cell sorter
PCR
ELISA

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