Differentiation of human embryonic stem cells into bipotent mesenchymal stem cells

Stem Cells
Emmanuel N OlivierEric E Bouhassira

Abstract

Mesenchymal stem cells (MSCs) are multipotent progenitors that can be found in many connective tissues, including fat, bone, cartilage, and muscle. We report here a method to reproducibly differentiate human embryonic stem cells (hESCs) into MSCs that does not require the use of any feeder layer. The cells obtained with this procedure are morphologically similar to bone marrow MSCs, are contact-inhibited, can be grown in culture for about 20 to 25 passages, have an immunophenotype similar to bone marrow MSCs (negative for CD34 and CD45 and positive for CD13, CD44, CD71, CD73, CD105, CD166, human leukocyte antigen [HLA]-ABC, and stage-specific embryonic antigen [SSEA]-4), can differentiate into osteocytes and adipocytes, and can be used as feeder cells to support the growth of undifferentiated hESCs. The ability to produce MSCs from hESCs should prove useful to produce large amounts of genetically identical and genetically modifiable MSCs that can be used to study the biology of MSCs and for therapeutic applications.

References

Sep 1, 1991·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·A I Caplan
Jan 1, 1988·Immunogenetics·B P WilliamsP N Goodfellow
Jul 26, 1996·Science·E SchröckT Ried
Nov 6, 1998·Science·J A ThomsonJ M Jones
Apr 2, 1999·Science·M F PittengerD R Marshak
Jun 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·D C ColterD J Prockop
Oct 16, 2002·Arteriosclerosis, Thrombosis, and Vascular Biology·Youichiro WadaTatsuhiko Kodama
Dec 17, 2002·Arthritis and Rheumatism·Elena A JonesDennis McGonagle
Nov 5, 2003·Stem Cells·Wilson A SilvaMarco A Zago
Nov 26, 2003·Blood Cells, Molecules & Diseases·Anne C RybickiRonald L Nagel
Feb 19, 2004·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Ichiro SekiyaDarwin J Prockop
May 4, 2004·The Journal of Laboratory and Clinical Medicine·Robert Chunhua ZhaoQin Han
May 18, 2004·Arthritis and Rheumatism·Saifeddin AlsalamehMartin Lotz
Jul 10, 2004·Circulation Research·Mark F Pittenger, Bradley J Martin
Jul 14, 2004·Yonsei Medical Journal·Jin Woo LeeSoo Bong Hahn
Aug 21, 2004·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Ryang Hwa LeeJin Sup Jung
Sep 3, 2004·Stem Cells·Yumi FukuchiKohichiro Tsuji
Sep 11, 2004·Biochemical and Biophysical Research Communications·Eun Ji GangHoeon Kim
Oct 20, 2004·Journal of Cellular and Molecular Medicine·D BakshR S Tuan
Dec 31, 2004·Stem Cells·B Linju YenYao-Chang Chen
Mar 1, 2005·Biochimie·A-M RodriguezC Dani
May 26, 2005·Journal of Dental Research·S R FacerG B Schneider
Jun 24, 2005·PLoS Medicine·Tiziano BarberiLorenz Studer

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Citations

Feb 17, 2009·Biogerontology·Jan O Nehlin, Torben Barington
Jan 17, 2012·Nature Cell Biology·Tim AhfeldtChad A Cowan
Dec 20, 2008·Proceedings of the National Academy of Sciences of the United States of America·Nathaniel S HwangJennifer Elisseeff
May 16, 2012·Proceedings of the National Academy of Sciences of the United States of America·Darja MaroltGordana Vunjak-Novakovic
Aug 12, 2010·Cellular Reprogramming·Premjit ArpornmaeklongPaul H Krebsbach
Nov 6, 2007·Rejuvenation Research·Nicholas R Forsyth, Jim McWhir
Sep 4, 2008·Stem Cells and Development·Nicholas R F Hannan, Ernst J Wolvetang
Nov 6, 2009·Stem Cells and Development·Anita L SørensenPhilippe Collas
Jul 2, 2010·Stem Cells and Development·Sergei A KuznetsovPamela Gehron Robey
Apr 5, 2011·Stem Cells and Development·Yao ChenWen Jie Zhang
May 23, 2012·Stem Cells and Development·Amer MahmoodMoustapha Kassem
Jun 27, 2012·Tissue Engineering. Part a·Hagit DomevJoseph Itskovitz-Eldor
Oct 13, 2007·Nucleic Acids Research·Jacob L GlassJohn M Greally
Sep 22, 2009·Genome Research·Romain DespratEric E Bouhassira
Aug 30, 2008·Cells, Tissues, Organs·Shelley E BrownPaul H Krebsbach
Jun 25, 2009·Arthritis Research & Therapy·Tracey E SwinglerIan M Clark
Jul 20, 2010·Stem Cell Research & Therapy·Darja MaroltGordana Vunjak Novakovic
Feb 23, 2011·Stem Cell Research & Therapy·Yulia ShamisJonathan A Garlick
May 1, 2010·Journal of Leukocyte Biology·Beata WinnickaLinda H Shapiro
Dec 17, 2008·Yi chuan = Hereditas·Shi-Min AnZhuo-Hua Zhang
Jan 1, 2010·Bioinformation·Andre BarkhordarianFrancesco Chiappelli
Aug 21, 2013·Tissue Engineering. Part B, Reviews·Aixin ChengSusan J Kimber

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