Differentiation of Human Induced-Pluripotent Stem Cells into Smooth-Muscle Cells: Two Novel Protocols

PloS One
Libang YangJianyi Zhang

Abstract

Conventional protocols for differentiating human induced-pluripotent stem cells (hiPSCs) into smooth-muscle cells (SMCs) can be inefficient and generally fail to yield cells with a specific SMC phenotype (i.e., contractile or synthetic SMCs). Here, we present two novel hiPSC-SMC differentiation protocols that yield SMCs with predominantly contractile or synthetic phenotypes. Flow cytometry analyses of smooth-muscle actin (SMA) expression indicated that ~45% of the cells obtained with each protocol assumed an SMC phenotype, and that the populations could be purified to ~95% via metabolic selection. Assessments of cellular mRNA and/or protein levels indicated that SMA, myosin heavy chain II, collagen 1, calponin, transgelin, connexin 43, and vimentin expression in the SMCs obtained via the Contractile SMC protocol and in SMCs differentiated via a traditional protocol were similar, while SMCs produced via the Sythetic SMC protocol expressed less calponin, more collagen 1, and more connexin 43. Differences were also observed in functional assessments of the two SMC populations: the two-dimensional surface area of Contractile SMCs declined more extensively (to 12% versus 44% of original size) in response to carbachol treatment, whil...Continue Reading

References

Nov 6, 1998·Science·J A ThomsonJ M Jones
Jul 23, 2004·Physiological Reviews·Gary K OwensBrian R Wamhoff
Jul 7, 2007·Netherlands Heart Journal : Monthly Journal of the Netherlands Society of Cardiology and the Netherlands Heart Foundation·S S M RensenG J J M van Eys
Apr 18, 2008·Development·Per WastesonPer Lindahl
Mar 26, 2010·Tissue Engineering. Part B, Reviews·Jeffrey A BeamishRoger E Marchant
Jul 21, 2010·Nature·K KimG Q Daley
Dec 7, 2013·Stem Cells Translational Medicine·Melanie MarchandRenee A Reijo Pera
Feb 11, 2014·Current Opinion in Chemical Engineering·Cristina E FernandezGeorge A Truskey
Oct 20, 2014·Nature Medicine·Carey L WatsonMichael A Helmrath
Nov 8, 2014·Stem Cells Translational Medicine·Sumati SundaramLaura Niklason
Jan 7, 2015·The Biochemical Journal·Biraja C DashYibing Qyang

❮ Previous
Next ❯

Citations

Dec 16, 2017·Cellular and Molecular Life Sciences : CMLS·Diana Klein
Sep 16, 2017·Pediatric Research·Philip M IannacconeArun K Sharma
Feb 14, 2018·Expert Opinion on Biological Therapy·Jakub SmolarDaniel Eberli
Feb 9, 2017·Development·Natasha AroraLinda G Griffith
Jul 7, 2018·Current Protocols in Human Genetics·Mingxia Gu
Oct 13, 2017·Circulation Research·Libang YangJianyi Zhang
Sep 2, 2017·Arteriosclerosis, Thrombosis, and Vascular Biology·Eithne Margaret MaguireQingbo Xu
Jan 29, 2019·Cardiovascular Research·Emily A PinheiroPaul W Burridge
Sep 5, 2018·Frontiers in Cardiovascular Medicine·Marianna TsifakiAndriana Margariti
Jul 22, 2016·Biotechnology Letters·Maryam PajooheshMasoud Soleimani
Aug 5, 2017·Journal of the American College of Cardiology·Mounica YanamandalaJianyi Zhang
Feb 15, 2020·Molecular Therapy. Methods & Clinical Development·Vera J MehlerMelanie L Moore
Mar 8, 2021·Stem Cell Research & Therapy·Anja TrillhaaseZouhair Aherrahrou
Mar 20, 2021·Stem Cell Reviews and Reports·Anja TrillhaaseJeanette Erdmann
May 6, 2021·American Journal of Physiology. Gastrointestinal and Liver Physiology·Magdalena KasendraMichael A Helmrath
Nov 19, 2020·ACS Biomaterials Science & Engineering·Kai WangYong Yang

❮ Previous
Next ❯

Methods Mentioned

BETA
SMA
flow-cytometry
FACS
Assay
ELISA
flow

Software Mentioned

SPSS
CellC

Related Concepts

Related Feeds

Cell Fate Conversion By mRNA

mRNA-based technology is being studied as a potential technology that could be used to reprogram cell fate. This technique provides the potential to generate safe reprogrammed cells that can be used for clinical applications. Here is the latest research on cell fate conversion by mRNA.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.