Differentiation of human iPSCs into functional podocytes

PloS One
Caroline RauchAnja Wilmes

Abstract

Podocytes play a critical role in glomerular barrier function, both in health and disease. However, in vivo terminally differentiated podocytes are difficult to be maintained in in vitro culture. Induced pluripotent stem cells (iPSCs) offer the unique possibility for directed differentiation into mature podocytes. The current differentiation protocol to generate iPSC-derived podocyte-like cells provides a robust and reproducible method to obtain podocyte-like cells after 10 days that can be employed in in vitro research and biomedical engineering. Previous published protocols were improved by testing varying differentiation media, growth factors, seeding densities, and time course conditions. Modifications were made to optimize and simplify the one-step differentiation procedure. In contrast to earlier protocols, adherent cells for differentiation were used, the use of fetal bovine serum (FBS) was reduced to a minimum, and thus ß-mercaptoethanol could be omitted. The plating densities of iPSC stocks as well as the seeding densities for differentiation cultures turned out to be a crucial parameter for differentiation results. Conditionally immortalized human podocytes served as reference controls. iPSC-derived podocyte-like cell...Continue Reading

References

Feb 22, 2003·Journal of the American Society of Nephrology : JASN·Jordan A Kreidberg
Jun 16, 2005·Kidney International·Michael R VaughanStuart J Shankland
Apr 21, 2006·Kidney International·K KatsuyaT Yamamoto
Apr 26, 2007·Kidney International·S J ShanklandP Mundel
May 16, 2007·Kidney & Blood Pressure Research·J KrtilT Zima
Sep 7, 2007·American Journal of Physiology. Renal Physiology·Grace M MituRaimund Hirschberg
Aug 14, 2008·Nature Reviews. Molecular Cell Biology·Shin-ichi NishikawaConcepcion R Nierras
Oct 20, 2009·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Noemi FusakiMamoru Hasegawa
Dec 4, 2009·American Journal of Physiology. Renal Physiology·Toru SakairiJeffrey B Kopp
Sep 11, 2010·Advanced Drug Delivery Reviews·Jan Willem LeeuwisRoel Goldschmeding
Mar 10, 2011·Nature Reviews. Genetics·Hao ZhuGeorge Q Daley
Apr 12, 2011·Nature Methods·Guokai ChenJames A Thomson
Jun 3, 2011·American Journal of Physiology. Renal Physiology·Seetharamaiah ChittiprolRichard F Ransom
Nov 8, 2011·Annual Review of Physiology·Anna Greka, Peter Mundel
Nov 15, 2011·Nature Reviews. Drug Discovery·Marica GrskovicGeorge Q Daley
Dec 8, 2011·Nature Medicine·Gustavo TiscorniaJuan Carlos Izpisúa Belmonte
Mar 20, 2012·Cell·Anne B C Cherry, George Q Daley
May 18, 2012·Nephrology·Lan NiPeter W Mathieson
Jun 19, 2012·Cell Stem Cell·Shinya Yamanaka
Oct 5, 2012·Nature Reviews. Molecular Cell Biology·Milena BellinChristine L Mummery
Jul 23, 2013·The International Journal of Developmental Biology·Shiho OedaMakoto Asashima
Sep 4, 2013·Nature Reviews. Nephrology·Florian GrahammerTobias B Huber
Dec 19, 2013·PloS One·Stefano Da SaccoLaura Perin
Feb 7, 2014·The EMBO Journal·Haruhisa InoueShinya Yamanaka
Feb 28, 2014·American Journal of Physiology. Renal Physiology·Evgenia DobrinskikhJudith Blaine
Jun 13, 2014·Genesis : the Journal of Genetics and Development·Paul T Kroeger, Rebecca A Wingert
Dec 2, 2014·Nature Biotechnology·Thorsten M SchlaegerGeorge Q Daley
Jan 8, 2015·Frontiers in Endocrinology·Janina Müller-Deile, Mario Schiffer
Feb 1, 2015·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Moin A Saleem
Feb 20, 2015·Current Opinion in Organ Transplantation·Albert Q Lam, Joseph V Bonventre
Apr 9, 2015·Frontiers in Medicine·Sandeep K Mallipattu, John Cijiang He
Apr 29, 2015·The Journal of Cell Biology·Rizaldy P Scott, Susan E Quaggin
Oct 13, 2015·Nature Biotechnology·Ryuji MorizaneJoseph V Bonventre
Oct 31, 2015·Journal of the American Society of Nephrology : JASN·Christodoulos XinarisGiuseppe Remuzzi
Jan 8, 2016·Biomarker Insights·Benjamin S Freedman

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Citations

Apr 23, 2020·Cells·Alok JhaPravin C Singhal
Aug 25, 2020·Stem Cells International·Patrícia de Carvalho RibeiroHeloisa Cristina Caldas
Dec 11, 2020·Stem Cell Research & Therapy·Patrícia de Carvalho RibeiroHeloisa Cristina Caldas
Dec 1, 2020·Frontiers in Physiology·Ricardo Romero-GuevaraChristodoulos Xinaris
Feb 26, 2021·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Sara WellensMaxime Culot
May 1, 2021·International Journal of Molecular Sciences·Quentin AlleJean-Marc Lemaitre
Sep 5, 2020·Chemical Reviews·Adam M JorgensenAnthony Atala
Oct 20, 2021·Clinica Chimica Acta; International Journal of Clinical Chemistry·Lingfeng Zeng, Cheuk-Chun Szeto

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Datasets Mentioned

BETA
AF5729

Methods Mentioned

BETA
transfection
PCR
fluorescence microscopy
Fluorescence

Software Mentioned

GeneMapper ID - X

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