PMID: 7518213May 28, 1994Paper

Differentiation of human mast cells from bone-marrow and cord-blood progenitor cells by factors produced by a mouse stromal cell line

Annals of the New York Academy of Sciences
Michel ArockD Thierry

Abstract

Human bone-marrow or cord-blood progenitors (i.e., CD34+ cells) are easily purified by immunological methods and can be cultured on normal human-bone-marrow stromal cells for limited periods of time. Under these culture conditions, the number of progenitors declines in a few weeks and these cells disappear completely in less than 8 weeks. This fact suggests that this culture system is deprived of growth factor(s) able to support the self-renewal of stem cells. We have developed the culture of immunomagnetically purified human-bone-marrow- or cord-blood-derived CD34+ cells on a supportive mouse lipoblastic stromal cell line, MS-5. The long-term survival of clonogenic cells was analyzed in these cultures and compared with the results obtained by culture on human-bone-marrow stromal cells. The results demonstrated that only coculture of CD34+ cells on MS-5 layers allows the survival of clonogenic progenitors for at least 12 weeks. Cytospin smears were regularly performed and cell morphology was examined after classical staining methods (i.e., M.G.G. and toluidine blue staining). Histologic analysis demonstrated the growth of mast-cell-like metachromatic cells after the second week of incubation on MS-5 layer. The highest percentag...Continue Reading

Citations

Jan 6, 1999·The Journal of Pathology·S ZhangW R Roche
Aug 23, 2001·International Immunopharmacology·M BidriM Arock
May 15, 2004·Journal of Clinical Periodontology·S SteinsvollK Schenck
Sep 1, 1996·Cancer Treatment Reviews·R M Weber-NordtR Mertelsmann
Mar 18, 2006·Experimental Hematology·Takashi YoshikuboHisafumi Okabe

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