Differentiation of human monocytes and derived subsets of macrophages and dendritic cells by the HLDA10 monoclonal antibody panel

Clinical & Translational Immunology
Anna Ohradanova-RepicHannes Stockinger

Abstract

The mononuclear phagocyte system, consisting of monocytes, macrophages and dendritic cells (DCs), has an important role in tissue homeostasis as well as in eliciting immune responses against invading pathogens. Blood monocytes have been viewed for decades as precursors of tissue macrophages. Although the newest data show that in the steady state resident macrophages of many organs are monocyte independent, blood monocytes critically contribute to tissue macrophage and DC pools upon inflammation. To better understand the relationship between these populations and their phenotype, we isolated and differentiated human blood CD14(+) monocytes in vitro into immature and mature monocyte-derived dendritic cells (MoDCs) as well as into seven different monocyte-derived macrophage subsets. We used the panel of 70 monoclonal antibodies (mAbs) submitted to the 10th Human Leukocyte Differentiation Antigen Workshop to determine the expression profiles of these 10 populations by flow cytometry. We now can compile subpanels of mAbs to differentiate the 10 monocyte/macrophage/MoDC subsets, providing the basis for novel diagnostic and therapeutic tools.

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Citations

Feb 6, 2017·Bioinformatics·Hao-Chih LeeBrian A Kidd
Oct 20, 2017·Human Molecular Genetics·Pinar MesciAlysson R Muotri
Jan 19, 2019·Journal of Leukocyte Biology·Anna Ohradanova-RepicVladimir Leksa
Mar 19, 2019·Frontiers in Immunology·Juliet R FooteAnthony W Segal
Apr 8, 2020·Frontiers in Immunology·Charles Caër, Mary Jo Wick
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Apr 10, 2020·Molecular Omics·Clément DelannoyElisabeth Elass-Rochard
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Nov 12, 2018·Biochimica Et Biophysica Acta. Gene Regulatory Mechanisms·Julita PietrzakAgnieszka Robaszkiewicz
Apr 24, 2021·Journal of Immunological Methods·Diane Bosman-SchluepRichard A J Janssen
Jun 11, 2021·The Journal of Allergy and Clinical Immunology·Karthi DuraisamyBilly K C Chow
Oct 19, 2021·Frontiers in Immunology·Dimitra LamprinakiNathalie Juge

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Datasets Mentioned

BETA
GM-CSF

Methods Mentioned

BETA
flow cytometry
density gradient centrifugation

Software Mentioned

FACSDiva
FlowJo

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