PMID: 9178096Jun 15, 1997Paper

Differentiation of human trophoblast cells in vitro is inhibited by dimethylsulfoxide

Journal of Cellular Biochemistry
T L Thirkill, G C Douglas

Abstract

Dimethyl sulfoxide (DMSO) exerts a number of biological effects, the most frequently cited being induction of cell differentiation. The compound also increases invasiveness and metastatic potential. In contrast to the many reports of DMSO-induced cell differentiation, we report here that DMSO inhibits the morphological differentiation of human cytotrophoblast cells to syncytiotrophoblast, as revealed by immunofluorescence staining for desmosomal protein and nuclei. Cytotrophoblast cells treated with DMSO under differentiation-inducing conditions remained mononucleated with intense desmosomals staining. The effect was dose dependent, with a maximal effect seen at 1.5% DMSO. Concentrations of < or = 0.5% had no effect and concentrations > 2% were cytotoxic. In addition to these morphological changes, DMSO inhibited secretion of human chorionic gonadotropin in a dose-dependent manner. At a concentration of 1.5%, DMSO inhibited secretion by 70%. If cytotrophoblast cells were cultured in the presence of DMSO and then switched to DMSO-free medium, they proceeded to differentiate normally. While the precise mechanism of action remains unknown, judicious use of DMSO may be a useful tool for studying and manipulating the differentiation...Continue Reading

References

May 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·S J CollinsR C Gallo
Feb 17, 1992·Biochimica Et Biophysica Acta·T J AnchordoguyL M Crowe
May 12, 1989·Journal of Immunological Methods·G C Douglas, B F King
Mar 1, 1995·In Vitro Cellular & Developmental Biology. Animal·A Yen, S Varvayanis
Mar 1, 1993·Placenta·G C Douglas, B F King

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Citations

Oct 1, 2009·Reproduction in Domestic Animals = Zuchthygiene·C H ChorescaS C Park
Jun 28, 2013·Biology of Reproduction·Jean-Francois MouilletYoel Sadovsky
Mar 25, 2004·Physiological Genomics·You-Hong ChengStuart Handwerger
Jan 7, 2021·Proceedings of the National Academy of Sciences of the United States of America·Xuan ShaoYan-Ling Wang
Jun 22, 2021·Frontiers in Cell and Developmental Biology·Tianjiao ChuYoel Sadovsky
Dec 15, 2005·Differentiation; Research in Biological Diversity·Namita SahgalMichael J Soares

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