Differentiation of human umbilical cord Wharton's jelly-derived mesenchymal stem cells into endometrial cells

Stem Cell Research & Therapy
Qin ShiYuquan Zhang

Abstract

Wharton's jelly-derived mesenchymal stem cells (WJ-MSCs) are a novel and promising strategy for tissue engineering because of their ability to differentiate into many cell types. We characterized the differentiation of WJ-MSCs into endometrial epithelial cell (EEC)-like and endometrial stromal cell (ESC)-like cells and assessed the effect of 17β-estradiol and 8-Br-cAMP on the differentiation system. WJ-MSCs were treated in two ways to differentiate into EEC-like and ESC-like cells respectively: cocultured with ESCs in control/differentiation medium (17β-estradiol, growth factors); and cultured in control/differentiation medium (8-Br-cAMP alone or 8-Br-cAMP plus 17β-estrogen and growth factors). Three signaling pathway inhibitors (SB203580, PD98059, H89) were used to investigate the mechanism of WJ-MSC differentiation into ESC-like cells. Immunofluorescence, western blot and flow cytometry analyses were used to analyze expression of epithelial markers and stromal cell markers. Enzyme-linked immunosorbent assays were used to test the production of secretory proteins associated with the differentiation of ESC-like cells. 17β-estradiol at 1 μM downregulated vimentin and CD13 and upregulated cytokeratin and CD9 proteins, promoting t...Continue Reading

References

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Citations

Jul 24, 2020·Regenerative Medicine·Fusanori YotsumotoShingo Miyamoto
May 24, 2020·Journal of Assisted Reproduction and Genetics·Ariel BenorAlan DeCherney
Jan 17, 2021·Life·Ilaria CampesiGiampiero Capobianco
Oct 15, 2020·Cells·Sahar EsfandyariAyman Al-Hendy
Jul 3, 2021·Cells·Sarama SahaSham S Kakar

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Methods Mentioned

BETA
flow cytometry
hysterectomy
enzyme-linked immunosorbent assay
ELISA

Software Mentioned

GraphPad Prism

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