Differentiation of infectious laryngotracheitis virus isolates by restriction fragment length polymorphic analysis of polymerase chain reaction products amplified from multiple genes

Avian Diseases
N C KirkpatrickAmir H Noormohammadi

Abstract

Infectious laryngotracheitis (ILT) has been identified in most countries around the world and remains a threat to the intensive poultry industry. Outbreaks of mild to moderate forms of ILT are common in commercial layer flocks, while sporadic outbreaks of ILT in broiler flocks have also been recognized as an emerging problem in several countries. Examination of viral isolates using restriction fragment length polymorphism of polymerase chain reaction (PCR-RFLP) from individual ILTV genes has suggested that some of these outbreaks were caused by vaccine strains. In this study, PCR-RFLP of a number of ILTV genes/genomic regions including gE, gG, TK, ICP4, ICP18.5, and open reading frame (ORF) B-TK was used to examine a number of historical and contemporary Australian ILTV isolates and vaccine strains. PCR-RFLP of gE using restriction endonuclease EaeI failed to distinguish between any of the isolates including the vaccine strains. PCR-RFLP of gG, TK, and ORFB-TK using restriction endonucleases MspI and FokI, respectively, divided all the isolates into two groups. PCR-RFLP of ICP18.5 and ICP4 using restriction endonuclease HaeIII separated the isolates into three different groups with some field isolates only able to be distinguis...Continue Reading

References

Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M Nei, W H Li
Sep 1, 1974·Australian Veterinary Journal·D A Purcell, P G Surman
Oct 16, 1965·The Veterinary Record·A E Churchill

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Citations

Nov 24, 2006·Avian Pathology : Journal of the W.V.P.A·Naomi C KirkpatrickAmir H Noormohammadi
May 31, 2013·Avian Pathology : Journal of the W.V.P.A·Mauricio J C CoppoJoanne M Devlin
Jul 17, 2012·Science·Sang-Won LeeJoanne M Devlin
Feb 18, 2011·Australian Veterinary Journal·H P BlackerA H Noormohammadi
Aug 1, 2011·Australian Veterinary Journal·H P BlackerA H Noormohammadi
Jan 28, 2014·Avian Pathology : Journal of the W.V.P.A·Kimberly R MenendezNathaniel L Tablante
May 26, 2016·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Rebecca Agnew-CrumptonAmir H Noormohammadi
Mar 10, 2016·Avian Pathology : Journal of the W.V.P.A·Eun-Jung ChoiSang-Won Lee
Feb 10, 2018·Journal of Immunoassay & Immunochemistry·Zeki ArasGökçenur Sanioğlu Gölen
Nov 1, 2013·World Journal of Virology·Shan-Chia Ou, Joseph J Giambrone
Feb 7, 2019·Avian Pathology : Journal of the W.V.P.A·Stephen J SpatzClaudio L Afonso
Feb 14, 2007·Veterinary Research·Walter FuchsThomas C Mettenleiter

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Methods Mentioned

BETA
PCR
electrophoresis

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