Differentiation of Lactobacillus delbrueckii subsp. bulgaricus and Lactobacillus delbrueckii subsp. lactis by SDS-PAGE of cell-wall proteins

Letters in Applied Microbiology
M GattiE Neviani

Abstract

In the present study, a method based on SDS-PAGE fingerprinting of surface layer proteins was developed to identify Lactobacillus delbrueckii subsp. bulgaricus and subsp. lactis dairy isolates. The two subspecies, identified by species-specific PCR, were characterized by different SDS-PAGE cell-wall protein profiles; subspecies bulgaricus showed one band of about 31 kDa which, in some cases, was observed at a doublet, and subspecies lactis showed one band of about 21 kDa or 18 kDa. The sensitivity of this procedure for discriminating between the two subspecies was very high. The different types of SDS-PAGE profile for cell-wall proteins of the strains studied in this work did not seem to be correlated to the different dairies of origin. The method appears to be an efficient taxonomic tool. It has the advantage of easy gel interpretation over fingerprinting of whole-cell protein extracts, and may be used as an alternative to established PCR-based techniques which, though rapid and safe, require expensive instruments and reagents.

References

Jan 7, 1998·Letters in Applied Microbiology·M GattiE Neviani
Jun 22, 1999·The Journal of Dairy Research·G Giraffa, D Mora
Jan 29, 2000·Journal of Bacteriology·M Sára, U B Sleytr
Aug 2, 2003·International Journal of Food Microbiology·Judith A Narvhus, Tendekayi Henry Gadaga
Jun 1, 1990·Applied and Environmental Microbiology·M DelleyH Hottinger

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Citations

Feb 6, 2003·International Journal of Food Microbiology·G GiraffaE Neviani
Mar 5, 2004·International Journal of Food Microbiology·G GiraffaE Neviani
Feb 3, 2009·International Journal of Food Microbiology·Stella L LonigroPaola Lavermicocca
Dec 27, 2011·International Journal of Systematic and Evolutionary Microbiology·Yuko KudoKoichi Watanabe
May 7, 2013·International Journal of Systematic and Evolutionary Microbiology·David B AdimpongLene Jespersen
Dec 24, 2010·Microbiology·Kana Tanigawa, Koichi Watanabe

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