Differentiation of Lactobacillus delbrueckii subspecies by ribotyping and amplified ribosomal DNA restriction analysis (ARDRA)

Journal of Applied Microbiology
V MitevaJ Mengaud


To differentiate the subspecies of Lactobacillus delbrueckii, subsp. delbrueckii, subsp. lactis and subsp. bulgaricus. Amplified ribosomal DNA restriction analysis (ARDRA) and ribotyping were applied to over 30 strains. Both methods analyse the ribosomal genes which carry useful information about the evolutionary and taxonomic relationship among bacteria. The methods proved to be reliable and highly reproducible. ARDRA was applied to 16S rDNA, 23S rDNA and the IGS region, thus covering the whole rrn operon with eight restriction enzymes. Only EcoRI differentiated Lact. delbrueckii subsp. bulgaricus from Lact. delbrueckii subsp. delbrueckii/Lact. delbrueckii subsp. lactis, which confirmed the finding of other authors. Ribotyping with different enzymes under precisely optimized conditions revealed a high level of strain polymorphism. Only ribotyping with EcoRI allowed differentiation of the three subspecies on the basis of typical hybridization patterns. The successful differentiation of the three subspecies of Lact. delbrueckii by EcoRI ribotyping offers a new possibility for precise identification and differentiation of strains and new isolates. Both methods could be used for differentiation of Lact. delbrueckii subspecies.


Jul 28, 2006·Antonie van Leeuwenhoek·Galina D StoyanchevaIvo Y Boudakov
Feb 6, 2003·International Journal of Food Microbiology·G GiraffaE Neviani
Mar 5, 2004·International Journal of Food Microbiology·G GiraffaE Neviani
Apr 11, 2008·Clinical Microbiology Reviews·Valérie BouchetRichard Goldstein
Aug 4, 2006·International Journal of Food Microbiology·Daniela M GuglielmottiAndrea Quiberoni
Dec 24, 2010·Microbiology·Kana Tanigawa, Koichi Watanabe
Dec 14, 2002·FEMS Microbiology Letters·Frederic ChavagnatMichael G Casey
Oct 27, 2016·Bulletin of Experimental Biology and Medicine·V N AfonyushkinE V Dudareva


Oct 1, 1992·The Journal of Applied Bacteriology·V I MitevaT Stefanova
Dec 1, 1991·The Journal of Applied Bacteriology·U M RodriguesM D Collins
Dec 1, 1991·FEMS Microbiology Letters·G KöhlerK H Schleifer
May 1, 1990·Applied and Environmental Microbiology·G W TannockK Pedersen
Sep 1, 1986·Annales De L'Institut Pasteur. Microbiology·F Grimont, P A Grimont
Feb 1, 1989·Applied and Environmental Microbiology·G W Tannock
May 1, 1996·The Journal of Applied Bacteriology·S Selenska-PobellA Squartini
Jul 2, 1998·Applied and Environmental Microbiology·W ZhongG Reid
Sep 19, 1998·Systematic and Applied Microbiology·V MitevaM Ljubenov
Sep 29, 1998·Journal of Applied Microbiology·G GiraffaM G Fortina
Jun 1, 1990·Applied and Environmental Microbiology·M DelleyH Hottinger

Related Concepts

Lactobacillus delbrueckii
Restriction Fragment Length Polymorphism
Enzymes, antithrombotic
Nucleic Acid Hybridization Procedure
DNA, Ribosomal
Deoxyribonuclease EcoRI
Cell Differentiation Process
Recombinant DNA

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Sexual Dimorphism in Neurodegeneration

There exist sex differences in neurodevelopmental and neurodegenerative disorders. For instance, multiple sclerosis is more common in women, whereas Parkinson’s disease is more common in men. Here is the latest research on sexual dimorphism in neurodegeneration

HLA Genetic Variation

HLA genetic variation has been found to confer risk for a wide variety of diseases. Identifying these associations and understanding their molecular mechanisms is ongoing and holds promise for the development of therapeutics. Find the latest research on HLA genetic variation here.

Super-resolution Microscopy

Super-resolution microscopy is the term commonly given to fluorescence microscopy techniques with resolutions that are not limited by the diffraction of light. Here are the latest discoveries pertaining to super-resolution microscopy.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.

Brain Lower Grade Glioma

Low grade gliomas in the brain form from oligodendrocytes and astrocytes and are the slowest-growing glioma in adults. Discover the latest research on these brain tumors here.

CD4/CD8 Signaling

Cluster of differentiation 4 and 8 (CD8 and CD8) are glycoproteins founds on the surface of immune cells. Here is the latest research on their role in cell signaling pathways.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.