Differentiation of mesenchymal stem cells under hypoxia and normoxia: lipid profiles revealed by time-of-flight secondary ion mass spectrometry and multivariate analysis

Analytical Chemistry
Nicole GeorgiMarcel Karperien

Abstract

Mesenchymal stem cells (MSC) have the ability to self-renew and differentiate into multiple cell types valuable for clinical treatment of rheumatic pathologies. To study the chondrogenic potential of MSC and identify the conditions that recreate the native cartilage environment, we used time-of-flight secondary ion mass spectrometry (TOF-SIMS) for label-free detection of cell-type- and environmental-condition-specific molecular profiles. We observed that coculture of human MSC and chondrocytes under standard culture conditions leads to improved extracellular matrix (ECM) deposition. In marked contrast, this effect was lost under low oxygen tension. This improved extracellular matrix deposition was associated with a significant decrease in lipids and in particular cholesterol under low oxygen tension as revealed by TOF-SIMS coupled to principal component analysis and discriminant analysis. We furthermore demonstrate that the higher cholesterol levels under normoxia might regulate fibroblast growth factor 1 (FGF-1) gene expression which was previously implemented in increased ECM production in the cocultures. In conclusion, our study shows an unexpected role of lipids as orchestrators of chondrogenesis in response to oxygen tensi...Continue Reading

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Citations

Nov 25, 2016·Nature Reviews. Rheumatology·Beatriz RochaFrancisco J Blanco
Oct 16, 2016·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Saba FarnaghiYin Xiao
Sep 28, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Martin R L PaineShane R Ellis
Jan 1, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Saba FarnaghiYin Xiao
Jun 17, 2021·Stem Cell Reviews and Reports·Daniela S C BispoAna M Gil
Jul 21, 2017·Analytical Chemistry·Brenda BakkerBerta Cillero-Pastor

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