Differentiation of monocytes to macrophages induced by influenza virus-infected apoptotic cells

The Journal of General Virology
Noboru UchideToshio Yamakawa

Abstract

The effect of the culture supernatant of influenza virus (IV)-infected apoptotic and non-apoptotic cells on the differentiation of monocytes to macrophages was investigated. IV infection induced apoptotic DNA fragmentation in cultured chorion cells but not in amnion cells prepared from human foetal membrane tissue. To examine the differentiation of monocytes to macrophages, an adhesion assay was employed using the human monocytic leukaemia THP-1 cell line. THP-1 cells became adherent to a substrate by incubation with the culture supernatant of IV-infected chorion cells, but not with that of amnion cells. The spreading THP-1 cells were morphologically characteristic of macrophages and they phagocytosed latex particles. RT-PCR analysis revealed that the expression of class A scavenger receptor mRNA was induced in THP-1 cells by incubation with the culture supernatant of IV-infected chorion cells. These results suggested that monocytic THP-1 cells were morphologically and functionally differentiated to macrophages by IV-infected apoptotic cells due to a soluble factor released from the apoptotic cells.

References

Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·A MatsumotoH Suzuki
Jan 1, 1989·Journal of Biochemistry·K HiranoT Osawa
Apr 17, 1986·Journal of Immunological Methods·T Oda, H Maeda
Jan 1, 1980·International Review of Cytology·A H WyllieA R Currie
Nov 1, 1995·The Journal of General Virology·I MoriY Kimura
Feb 3, 2000·Mechanisms of Ageing and Development·H ShiraiT Kodama

❮ Previous
Next ❯

Citations

Aug 5, 2014·Cellular Immunology·Thomas Myles AshhurstNicholas Jonathan Cole King
Jun 6, 2014·Reproductive Sciences·Renju S RajMark Phillippe
Sep 10, 2003·International Reviews of Immunology·R Joel Lowy
Nov 24, 2017·Scientific Reports·S F KhaiboullinaS C Verma
Feb 6, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yumi HashimotoYoshinobu Nakanishi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.