Differentiation of mumps virus strains with monoclonal antibody to the HN glycoprotein.

Infection and Immunity
A C ServerJ S Wolinsky

Abstract

A hybridoma cell line secreting antibody of the immunoglobulin G3 isotype with kappa light chains and with activity against the HN glycoprotein of the Kilham strain of mumps virus was established. The antibody exhibited structural homogeneity in sodium dodecyl sulfate-polyacrylamide gel electrophoresis and had a microheterogeneous isoelectric spectrum characteristic of an antibody of monoclonal origin. The specificity of the monoclonal antibody, shown by immunoprecipitation performed with radiolabeled virus and infected cell lysates, was for the larger mumps virus glycoprotein. In functional assays the antibody inhibited the hemagglutinating and neuraminidase activities and neutralized the infectivity of the homologous Kilham strain of virus and clearly differentiated this strain from two heterologous strains, Enders and O'Take. The antibody was markedly less effective with the O'Take strain than with either the Kilham or Enders strain in inhibiting both hemagglutination and neuraminidase activity against the macromolecular substrate fetuin. The inhibition of the neuraminidase activity of the Kilham strain was independent of substrate size, the antibody inhibiting the hydrolysis of both fetuin and the trisaccharide neuraminlact...Continue Reading

References

Jan 1, 1976·Virology·R A LambP W Choppin
Dec 1, 1978·The Journal of General Virology·C Orvell
Jan 1, 1979·Intervirology·J M Hardwick, R H Bussell
Jan 1, 1979·Archives of Virology·R Leprat, M Aymard
Dec 1, 1978·The Journal of General Virology·C Orvell
Feb 1, 1976·Journal of Virology·S C Jensik, S Silver
Jun 15, 1973·Annals of the New York Academy of Sciences·A R WilliamsonH W Kreth
Jul 1, 1974·European Journal of Biochemistry·W M Bonner, R A Laskey
Feb 1, 1980·The Journal of General Virology·B K RimaS J Martin
Jun 1, 1981·Annals of Neurology·P K Coyle, J S Wolinsky
Jan 1, 1980·The Journal of General Virology·M McCarthy, R T Johnson
Mar 1, 1955·Proceedings of the Society for Experimental Biology and Medicine·V J CABASSO, H R COX
Jan 1, 1949·Acta Pathologica Et Microbiologica Scandinavica·O OUCHTERLONY

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Citations

Jan 1, 1989·Microbiology and Immunology·R YamaguchiK Ueda
Jan 1, 1989·Archives of Virology·A NathJ S Wolinsky
Aug 3, 2013·Microbiology and Immunology·Masaaki MatsuuraYasuko Mori
Oct 31, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Gustavo H Dayan, Steven Rubin
Oct 1, 1984·European Journal of Clinical Microbiology·R A Polin
Jan 19, 1984·The New England Journal of Medicine·R T JohnsonA Vaisberg
Sep 19, 2002·The Journal of General Virology·Claes ÖrvellAgneta Samuelson
Jan 25, 1988·Biochimica Et Biophysica Acta·N SatoA Kimura
Apr 15, 1983·Virology·M N Waxham, J S Wolinsky

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