Differentiation of murine committed megakaryocytic progenitors isolated by a novel strategy reveals the complexity of GATA and Ets factor involvement in megakaryocytopoiesis and an unexpected potential role for GATA-6.

Experimental Hematology
Stéphanie DumonJon Frampton

Abstract

The differentiation of megakaryocytes is characterized by polyploidization and cytoplasmic maturation leading to platelet production. Studying these processes is hindered by the paucity of bone marrow megakaryocytes and their precursors. We describe a method for the expansion and purification of committed megakaryocyte progenitors and demonstrate their usefulness by studying changes in the expression of Ets and GATA family transcription factors throughout megakaryocytopoiesis. A two-step serum-free method was developed. Cells isolated using this method were analyzed for surface marker expression by flow cytometry, and for their ability to differentiate using single-cell culture. Purified progenitors were induced to differentiate and analyzed with respect to their ploidy by flow cytometry and expression of specific genes by RT-PCR. A population of Lin- c-kit+ CD45+ CD41+ CD31+ CD34low CD9low FcgammaRII/IIIlow Sca-1med/low committed megakaryocyte progenitors was purified. These cells could be differentiated efficiently, achieving ploidy of up to 128N. Analysis of RNA demonstrated the expected increases in expression of key megakaryocyte-associated genes. RT-PCR analysis also revealed that a range of Ets and GATA factors are expre...Continue Reading

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Citations

Mar 10, 2011·Blood·Alexandra MazharianSteve P Watson
Mar 20, 2009·Development·Tessa PeterkinRoger Patient
Feb 26, 2015·Journal of Thrombosis and Haemostasis : JTH·K JimenezC Gasche
Sep 19, 2015·PloS One·Giacomo VolpeStéphanie Dumon
Mar 8, 2008·American Journal of Respiratory Cell and Molecular Biology·Katrin AhlbrechtRobert Voswinckel

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