Differentiation of myeloid cells in liquid culture: 2. Acute myelocytic leukemia cells

Cell Biochemistry and Function
E Archimbaud, H D Preisler

Abstract

In a companion paper we demonstrated that normal peripheral blood granulocytic precursor cells differentiate after 2-3 weeks in suspension culture. In the studies described here leukemic blast cells obtained from 14 patients with acute myelocytic leukemia (AML) and two patients with chronic myelocytic leukemia in blastic crisis were cultured in McCoy's 5A medium containing 15 per cent fetal bovine serum for 2-3 weeks at 37 degrees C in an atmosphere of 5 per cent CO2-95 per cent room air. 'Spontaneous' myeloid differentiation (20 x 10(4) viable mature myeloid cells ml-1) occurred in the cultures of cells obtained from 8 pts. The differentiation was granulocytic in three cases, monocytic in four cases and of mixed type in one case. Differentiation was independent of the growth of the cells in culture and occurred in four cases after the first week. Monocytic differentiation was seen only in AML of the FAB M4 type whereas granulocytic or mixed differentiation were seen only in AML of the FAB M1 or M2 types. When PHA leucocyte conditioned medium (PHA-LCM) was added to the cultures monocytic/macrophage differentiation was favoured. Inducers of the differentiation of the HL-60 cell line (N-methylacetamide, cytosine arabinoside, or r...Continue Reading

References

Nov 1, 1979·British Journal of Cancer·G PalúP Alexander
Jun 1, 1975·Cell Differentiation·H D Preisler, G Lyman
Oct 1, 1986·Cell Biochemistry and Function·E Archimbaud, H D Preisler
May 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·T R BreitmanS J Collins
Apr 1, 1981·American Journal of Clinical Pathology·J Kirshner, J Goldberg

Related Concepts

Marrow
Cell Differentiation Process
Cell Division Phases
Leukemia, Monocytic, Chronic
Acute Myeloid Leukemia, M1

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