PMID: 9556026Apr 29, 1998Paper

Differentiation of oligodendrocyte occurs in contact with astrocyte

Journal of Neuroscience Research
Y SakuraiH Asou

Abstract

We have generated stage-specific oligodendrocytes, which are identified as immature oligodendrocytes by the expression of specific markers. These cells were generated by using 3 types of intrinsic and extrinsic factors for influencing the characteristics of oligodendrocyte-lineage cells. The factors included 10% serum in the medium to induce cell proliferation, bFGF in serum-free medium to increase survival and induce differentiation, and 0.25% trypsin to remove nonoligodendrocyte lineage cells. We have also demonstrated that it is possible to supply a huge amount of homogeneous populations of stage-specific immature oligodendrocytes after 35 days' culture. The maturation of these cells is controlled by interacting with astrocytes in vitro, resulting in the expression of MBP isoforms (14.0 and 18.5 kDa) that may participate in mature oligodendrocyte membrane compaction.

Citations

Aug 24, 2016·Frontiers in Cell and Developmental Biology·Helena S DominguesJoão B Relvas
Jul 19, 2002·Journal of Neuroscience Research·Chika SeiwaHiroaki Asou
Mar 18, 2020·Frontiers in Cellular Neuroscience·Remi RonzanoAnne Desmazieres
Apr 15, 2004·Molecular and Cellular Neurosciences·Raghuram SelvarajuUrsula Boschert

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