Differentiation of oligodendrocytes cultured from developing rat brain is enhanced by exogenous GM3 ganglioside

Journal of Neuroscience Research
S H YimR H Quarles

Abstract

Cultures consisting primarily of O-2A progenitor cells and immature oligodendrocytes with a few microglia and astrocytes were obtained by shaking primary cultures from neonatal rat brain after 12-14 days in vitro. Addition of 50 micrograms/ml exogenous Neu-NAc alpha 2-3Gal beta 1-4Glc beta 1-1'ceramide (GM3 ganglioside) to the cultures resulted in an increase in the number and thickness of cell processes that stained intensely for sulfatide and galactocerebroside (galC) in comparison to control cultures without added GM3. The treated cultures also contained fewer astrocytes than control cultures as revealed by immunostaining for glial fibrillary acidic protein (GFAP). Cells that immunostained for both GFAP and sulfatide/galC were very rare in control cultures but were frequently seen in the GM3-treated cultures, suggesting that these may represent cells changing their direction of differentiation away from type II astrocytes toward oligodendrocytes under the influence of GM3. These effects on the developing rat oligodendrocytes were specific for GM3 ganglioside and were not produced by adding GM1, GM2, GD3, or GD1a to the cultures. Lactosyl ceramide and neuraminyl lactose were also ineffective. When control cultures were initia...Continue Reading

References

Jul 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·B S Hilbush, J M Levine
Jan 1, 1991·Developmental Neuroscience·L L Bambrick, P E Braun
Jun 1, 1991·Journal of Neurochemistry·G S ShuklaN S Radin
Sep 1, 1990·The Journal of Cell Biology·R ArmstrongM Dubois-Dalcq
Aug 1, 1989·Journal of Neuroscience Research·H Z ShengP F Bartlett
Nov 30, 1989·Biochemical and Biophysical Research Communications·K F Chan
Nov 1, 1989·The Journal of Cell Biology·C GodfraindM Dubois-Dalcq
Apr 1, 1985·Journal of Neurochemistry·J R GoldenringR J DeLorenzo
Jun 1, 1989·Journal of Neuroscience Research·R K YuR W Ledeen
Jan 1, 1989·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·F BesnardG Labourdette
Mar 1, 1988·The Journal of Cell Biology·L FacciA Leon
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·R P Saneto, J de Vellis
Nov 15, 1988·The Journal of Comparative Neurology·S M LeVine, J E Goldman
May 19, 1987·Brain Research·W M CarrollF L Mastaglia
Feb 1, 1988·Journal of Neuroscience Research·V W YongS U Kim
Aug 1, 1985·Brain Research·P A Eccleston, D H Silberberg
Jan 1, 1986·Journal of Cellular Physiology·R Katoh-SembaS Varon
Oct 1, 1981·Neurochemical Research·R H QuarlesN H Sternberger
Jun 1, 1980·The Journal of Cell Biology·K D McCarthy, J de Vellis
Jan 1, 1982·The Journal of Membrane Biology·P H Fishman
Apr 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·B RanschtW Seifert
Jul 1, 1980·Journal of Neurochemistry·M A Williams, R H McCluer
Apr 1, 1993·Journal of Neuroscience Research·K Asotra, W B Macklin
May 1, 1961·The Journal of Biophysical and Biochemical Cytology·M B BUNGEH RIS

❮ Previous
Next ❯

Citations

Oct 1, 1995·Journal of Neurocytology·B Q KreiderD Pleasure
Feb 1, 1996·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·M StojiljkovićL Rakić
Sep 12, 1995·Proceedings of the National Academy of Sciences of the United States of America·G ZengR K Yu
Dec 7, 2013·Multiple Sclerosis International·Judith M Greer
Mar 25, 2008·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Kidsadagon PringproaKonstantin Wewetzer
Apr 22, 2009·Journal of Neuroscience Research·Edina SilajdzićSusan C Barnett
Sep 1, 2004·Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia·Michael P Pender
Mar 22, 2001·Experimental Neurology·E N NollP M Black
Jun 10, 2015·Proceedings of the National Academy of Sciences of the United States of America·James C DodgeLamya S Shihabuddin
Jun 13, 2020·Frontiers in Neurology·Alice V Alessenko, Elisabetta Albi
Feb 4, 2005·Journal of Neuro-oncology·Yasunori FujimotoToshiki Yoshimine
Feb 13, 2021·Natural Product Reports·Luise NowackRyan Gilmour
Aug 14, 1997·Biochimica Et Biophysica Acta·J A Mahoney, R L Schnaar
Jan 24, 2018·ACS Chemical Neuroscience·Tobias J KieserRyan Gilmour
Sep 6, 2021·Zhurnal nevrologii i psikhiatrii imeni S.S. Korsakova·A V AlessenkoA A Ustyugov

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.