Differentiation of primary human submandibular gland cells cultured on basement membrane extract

Tissue Engineering. Part a
Vanda SzlávikJanos Vag

Abstract

There is no effective treatment for the loss of functional salivary tissue after irradiation for head and neck cancer or the autoimmune disease Sjögren's syndrome. One possible approach is the regeneration of salivary glands from stem cells. The present study aimed to investigate whether small pieces of human submandiblar gland tissue contain elements necessary for the reconstruction of salivary rudiments in vitro via acinar and ductal cell differentiation. Primary submandibular gland (primary total human salivary gland; PTHSG) cells were isolated from human tissue and cultured in vitro using a new method in which single cells form an expanding epithelial monolayer on plastic substrates. Differentiation, morphology, number, and organization of these cells were then followed on basement membrane extract (BME) using RNA quantitation (amylase, claudin-1 (CLN1), CLN3, kallikrein, vimentin), immunohistochemistry (amylase and occludin), viability assay, and videomicroscopy. On the surface of BME, PTHSG cells formed acinotubular structures within 24 h, did not proliferate, and stained for amylase. In cultures derived from half of the donors, the acinar markers amylase and CLN3 were upregulated. The PTHSG culture model suggests that hu...Continue Reading

References

Jul 1, 1971·Archives of Otolaryngology·L R Eversole, W R Sabes
Dec 31, 1998·Gastroenterology·M Bjerknes, H Cheng
Nov 2, 2001·Nature·A SpradlingT Kai
Aug 12, 2003·Proceedings of the National Academy of Sciences of the United States of America·Li XinOwen N Witte
Apr 6, 2004·Developmental Biology·Tina JaskollMichael Melnick
Jul 22, 2004·Journal of Dermatological Science·Sang Woong YounKyoung Chan Park
Nov 24, 2004·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Carlton G BellowsJohan N M Heersche
Dec 27, 2005·Biochemical and Biophysical Research Communications·Teruki KishiHideki Taniguchi
Jan 10, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Mark DenhamRichard Mollard
May 27, 2006·European Cells & Materials·D WideraB Kaltschmidt
Jun 15, 2006·Proceedings of the National Academy of Sciences of the United States of America·Thai-Yen LingJohn Yu
Jul 29, 2006·Development, Growth & Differentiation·Mohamadreza Baghaban EslaminejadMohamad Massumi
Aug 19, 2006·Differentiation; Research in Biological Diversity·Vaishali N PatelMatthew P Hoffman
Oct 10, 2006·Experimental Cell Research·Wuyi KongH Peter Lorenz
Dec 13, 2006·Cells, Tissues, Organs·Ulrich Reinhart GoesslerFrank Riedel
Dec 23, 2006·Proceedings of the National Academy of Sciences of the United States of America·Devon A LawsonOwen N Witte
Feb 3, 2007·Current Opinion in Gastroenterology·Deborah C Rubin
Feb 24, 2007·Differentiation; Research in Biological Diversity·Sung-Won ChoHan-Sung Jung
Mar 10, 2007·Journal of Cellular Physiology·Janos VagBrian C O'Connell

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Citations

Feb 2, 2010·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·Swati PradhanRobert L Witt
Apr 23, 2009·Tissue Engineering. Part a·Swati PradhanMary C Farach-Carson
Dec 30, 2010·Tissue Engineering. Part a·Ola M MariaSimon D Tran
Aug 3, 2010·Tissue Engineering. Part C, Methods·Adi YanivAaron Palmon
Feb 26, 2010·Journal of Biomedicine & Biotechnology·Olga J Baker
Oct 15, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Alice BanhQuynh-Thu Le
Jul 23, 2015·Tissue Engineering. Part C, Methods·Orsolya HegyesiGábor Varga
Jan 4, 2017·Journal of Dental Research·H S ShinJ Y Lim
Jan 23, 2016·Journal of Dental Research·E BoriG Varga
May 26, 2018·Proceedings of the National Academy of Sciences of the United States of America·Julie P SaikiDaria Mochly-Rosen
Nov 2, 2018·Archives of Oral Biology·Jomy J VargheseDanielle S W Benoit

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Methods Mentioned

BETA
FCS
light microscopy
PCR
flow cytometry

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