Differentiation of respiratory syncytial virus subgroups with cDNA probes in a nucleic acid hybridization assay.

Journal of Clinical Microbiology
W M SullenderG W Wertz

Abstract

A new approach to respiratory syncytial (RS) virus subgroup determination was developed by using a simple nucleic acid filter hybridization technique. By this method, virus-infected cells are bound and fixed in a single step, and the viral RNA in the fixed-cell preparation is characterized directly by its ability to hybridize to cDNA probes specific for either the A or B subgroups of RS virus. The subgroup-specific probes were constructed from cDNA clones that corresponded to a portion of the extracellular domain of the RS virus G protein of either a subgroup B RS virus (8/60) or a subgroup A RS virus (A2). The cDNA probes were labeled with 32P and used to analyze RS virus isolates collected over a period of three decades. Replicate templates of infected cell preparations were hybridized with either the subgroup A or B probe. The subgroup assignments of 40 viruses tested by nucleic acid hybridization were in agreement with the results of subgroup determinations based on their reactivities with monoclonal antibodies, which previously has been the only method available for determining the subgroup classification of RS virus isolates. The nucleic acid hybridization assay has the advantage of providing broad-based discrimination of...Continue Reading

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Citations

Oct 1, 1996·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·G Mlinaric-GalinovicE E Walsh
Jul 9, 2010·Journal of Clinical Microbiology·Isolde C DapatHiroshi Suzuki
Jan 11, 2000·Clinical Microbiology Reviews·W M Sullender
May 1, 1993·Journal of Clinical Microbiology·W M SullenderL J Anderson
Feb 1, 1993·Current Problems in Pediatrics·O Ruuskanen, P L Ogra
Apr 1, 1992·Journal of Clinical Microbiology·P WalpitaJ D Connor

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