PMID: 8956489Jan 1, 1996Paper

Differentiation of Rhodococcus species by ribotyping

Journal of Basic Microbiology
S Jorks

Abstract

The ribosomal RNA gene restriction patterns (ribotyping) was investigated with respect to the characterization of Rhodococcus species and R. rhodochrous strains. Chromosomal DNA was prepared, digested with BamH I, blotted, and hybridized with acetylaminofluorene-labelled 16 + 23S rRNA from E. coli. The type strains of seven Rhodococcus species studied gave different hybridization patterns in each case. All strains tested were clearly distinguishable by ribotyping. Patterns contained two to eight bands between 1.4 kb and 11.8 kb and demonstrated the high genetic divergence of genus Rhodococcus. Investigation of nine R. rhodochrous strains resulted in patterns with seven or eight bands. One fragment, 4.4 kb in size, was common to all R. rhodochrous strains and appears to be characteristic of R. rhodochrous. Ribotyping was evaluated as a tool for distinguishing between Rhodococcus species.

References

Jan 1, 1992·Annual Review of Microbiology·W R Finnerty
Sep 1, 1986·Annales De L'Institut Pasteur. Microbiology·F Grimont, P A Grimont
Aug 1, 1988·Journal of General Microbiology·N A SaundersA G Taylor
Jul 1, 1989·Journal of General Microbiology·F M Thomson-CarterT H Pennington
Oct 1, 1988·Journal of General Microbiology·J Zakrzewska-CzerwińskaM Goodfellow
Oct 1, 1993·Applied and Environmental Microbiology·S Rodtong, G W Tannock
Nov 1, 1993·Applied and Environmental Microbiology·J E Olsen, J L Larsen
Jan 1, 1995·Applied and Environmental Microbiology·A DalsgaardH H Huss
Jan 1, 1995·International Journal of Systematic Bacteriology·F A RaineyE Stackebrandt
Mar 1, 1993·Applied and Environmental Microbiology·Y BerthierM Lemattre
Mar 1, 1995·Applied and Environmental Microbiology·C BragardH Maraite

❮ Previous
Next ❯

Citations

Apr 3, 2004·Research in Microbiology·Gwénola LefresnePatrick A D Grimont
Dec 3, 1999·Journal of Applied Microbiology·K S BellN Christofi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.