Differentiation of the HaCaT keratinocyte cell line: modulation by adrenomedullin

The British Journal of Dermatology
O Pleguezuelos, Supriya Kapas

Abstract

Adrenomedullin (AM) is a multifunctional peptide produced by a wide variety of cells, including keratinocytes. We, and others, have demonstrated that AM has a role as a growth regulatory factor of the skin and contributes as an antimicrobial agent in the integument's protective barrier. It is not known whether AM has a role in differentiating keratinocytes. To study the role of AM in keratinocyte differentiation, modulating the effects of calcium and in addition, to assess whether differentiated keratinocytes are still capable of initiating an inflammatory response. HaCaT cells were differentiated using CaCl2. Expression of transglutaminase type 1 (TG1) and E2F1 genes was used to monitor differentiation. AM secretion was measured by enzyme-linked immunosorbent assay (ELISA). NF-kappaB activity and interleukin (IL)-6 secretion in the cells were assessed after exposure to calcium and AM by electrophoretic mobility shift assay and ELISA, respectively. Secretion of AM by the keratinocyte cell line HaCaT was found to be increased during 1 mmol L(-1) CaCl2-induced cell differentiation but not 0.1 mmol L(-1) CaCl2. All treatments showed low levels of the cell proliferation marker, E2F1. Over time, cells incubated in the presence of 0....Continue Reading

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Citations

Jun 12, 2012·Mediators of Inflammation·Elena TaleroSalvatore Cuzzocrea
Mar 22, 2007·Expert Opinion on Biological Therapy·Nieves VarelaMario Delgado
Apr 8, 2010·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ludovic MicallefAlain Simon

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