PMID: 11932930Apr 5, 2002Paper

Differentiation of tyrosine hydroxylase-synthesizing and/or aromatic L-amino acid decarboxylase-synthesizing neurons in the rat mediobasal hypothalamus: quantitative double-immunofluorescence study

The Journal of Comparative Neurology
P V ErshovJean Thibault

Abstract

In this double-immunofluorescence study, we first quantified the neurons of the arcuate nucleus as immunoreactive (+) for tyrosine hydroxylase (TH) and/or aromatic L-amino acid decarboxylase (AADC) in rats at embryonic day 21 (E21), at postnatal day 9 (P9), and in adulthood by using conventional fluorescent or confocal microscopy. On E21, monoenzymatic (TH(+)AADC immunonegative (-) and TH(-)AADC(+)) neurons and bienzymatic (TH(+)AADC(+)) neurons accounted for 99% and 1%, respectively, of the whole neuron population expressing enzymes of dopamine synthesis. Further development was characterized by the dramatic increase in TH(+)AADC(-) dorsomedial and TH(+)AADC(+) dorsomedial populations from E21 to P9 as well as by the increase in the TH(+)AADC(+) dorsomedial population (in females) and a drop in the TH(+)AADC(-) ventrolateral and TH(+)AADC(-) dorsomedial (in males) populations from P9 to adulthood. In contrast to TH(+)AADC(-) (in males) and TH(+)AADC(+) neurons, the TH(-)AADC(+) neurons did not change in number from E21 to adulthood. Thus, in rat fetuses, the neurons synthesizing TH and/or AADC were mainly monoenzymatic, whereas during postnatal life the fraction of bienzymatic neurons increased by up to 60%.

References

May 1, 1990·Brain Research. Developmental Brain Research·I ReisertC Pilgrim
Dec 10, 1986·Brain Research·S DaikokuI Nagatsu
Dec 19, 1988·Neuroscience Letters·H OkamuraM Geffard
Oct 1, 1986·Physiology & Behavior·J P Kroon, A L Riley
Jul 1, 1982·Brain Research Bulletin·J M LauderK McCarthy
Jan 1, 1996·The Journal of Comparative Neurology·R E Coggeshall, H A Lekan
Apr 26, 1996·Neuroreport·W MurrellA Mackay-Sim
Aug 1, 1996·Progress in Neurobiology·Y MisuH Okamura
Apr 1, 1998·Proceedings of the Society for Experimental Biology and Medicine·P S MohanKumarJ L Voogt
Aug 5, 1998·The American Journal of Physiology·M A Vieira-Coelho, P Soares-Da-Silva
Oct 3, 1998·European Journal of Endocrinology·V I MelnikovaM V Ugrumov
Nov 13, 1998·Current Opinion in Neurobiology·H A Cameron, R McKay
Mar 2, 1999·Neuroscience·V MelnikovaM Ugrumov
Sep 1, 1964·Acta Physiologica Scandinavica·K JOHANSEN
Feb 1, 1946·The Anatomical Record·M ABERCROMBIE

❮ Previous
Next ❯

Citations

Mar 11, 2009·Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology·Varvara E DyakonovaDmitri A Sakharov
Apr 11, 2009·Journal de la Société de biologie·Mikhail V Ugrumov
Nov 7, 2012·Doklady Biological Sciences : Proceedings of the Academy of Sciences of the USSR, Biological Sciences Sections·V I MelnikovaM V Ugrumov
Apr 17, 2008·Zhurnal evoliutsionnoĭ biokhimii i fiziologii·G Z ZiiazetdinovaM V Ugriumov
Aug 25, 2015·Neuropharmacology·Roberto De LucaOlga A Sergeeva
Jun 10, 2011·Expert Review of Neurotherapeutics·Witold PolanskiGabriele Gille
Sep 1, 2005·Neuroscience and Behavioral Physiology·V I Mel'nikovaM V Ugryumov
Jul 16, 2016·Doklady. Biochemistry and Biophysics·A U KurinaM V Ugrumov

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.