Differentiation of underivatized monosaccharides by atmospheric pressure chemical ionization quadrupole time-of-flight mass spectrometry

Rapid Communications in Mass Spectrometry : RCM
Zhenqian ZhuJohn E Bartmess

Abstract

Differentiation of underivatized monosaccharides is essential in the structural elucidation of oligosaccharides which are closely involved in many life processes. So far, such differentiation has been usually achieved by electrospray ionization mass spectrometry (ESI-MS). As an alternative to ESI-MS, atmospheric pressure chemical ionization mass spectrometry (APCI-MS) should provide complementary results. A quadrupole time-of-flight (QTOF) mass spectrometer with accurate mass measurement ability was used with an APCI heated nebulizer ion source because we believe that a recently published article using a single quadrupole mass spectrometer assigned incorrect identities for APCI ions from hexoses. Using APCI-QTOF, the MS(2) and pseudo-MS(3) mass spectra of 11 underivatized monosaccharides were obtained under various collision voltages. The mass spectra were carefully interpreted after accurate mass measurement. Differentiation of three hexoses was achieved by different MS(2) spectra of their [M + NH(4)](+) and [M - H](-) ions. The MS(2) spectra of the [M + NH(4)](+) ions were also used to distinguish methyl α-D-glucose and methyl β-D-glucose, while the pseudo-MS(3) spectra of the [M + H](+) ions were utilized to differentiate th...Continue Reading

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