PMID: 9654192Jul 8, 1998Paper

Differentiation, proliferation and apoptosis levels in human leiomyoma and leiomyosarcoma

Journal of Cancer Research and Clinical Oncology
M T ValentiS Sartore

Abstract

A comparative analysis of the differentiation pattern, the proliferative behaviour, and the level of apoptosis between human benign and malignant neoplasms of smooth-muscle (SM) tissue is lacking. The clinical, histopathological, immunochemical, and immunocytochemical features of leiomyomas (LM) and leiomyosarcomas (LMS) were investigated by a panel of monoclonal antibodies specific for some differentiation markers of SM tissue (SM myosin and alpha-actin, desmin, and SM22) and for markers of non-muscle tissue (vimentin and non-muscle myosin). Proliferating normal and neoplastic cells were identified by proliferating-cell nuclear antigen (PCNA)/Ki67 immunostainings and the apoptotic cells were revealed by means of the terminal-deoxynucleotidyltransferase-mediated dUTP nick-end labelling technique. Gel electrophoresis and Western blotting, performed with anti-(SM1/SM2 myosin isoform) antibody, indicated quantitative differences between LMS and LM, which mirrored higher positive to negative nuclear ratios for PCNA, Ki67 and apoptosis in malignant as opposed to benign neoplasms. With LM, however, a similar SM1 to SM2 ratio could be associated with different proliferation levels. Uterine, gastric and intestinal LMS displayed specifi...Continue Reading

Citations

Oct 14, 2003·Journal of Comparative Pathology·G BettiniP S Marcato
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Nathan D PriceWei Zhang
Sep 19, 2012·ANZ Journal of Surgery·Marcos Vinicius PeriniChristopher Christophi
Jan 8, 2019·F1000Research·Stephen M SchwartzMark W Majesky
Jun 13, 2018·Scientific Reports·Yusuke SuzukiMichio Asahi

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