PMID: 2115338Jun 1, 1990Paper

Differentiation to a neuronal phenotype in bovine chromaffin cells is repressed by protein kinase C and is not dependent on c-fos oncoproteins

DNA and Cell Biology
B A DemeneixJ P Loeffler

Abstract

We investigated the intracellular signals underlying the neurotrophic response of adult bovine chromaffin cells to histamine and basic fibroblast growth factor (bFGF). Histamine produced significant neurite outgrowth within 48 hr, whereas the response to bFGF developed after 1 week. H7, a protein kinase C (PKC) inhibitor potentiated both the histamine and the bFGF responses, while another PKC antagonist, staurosporine, induced a rapid and efficient differentiation response when applied alone. These observations suggest that basal PKC activity is required for stabilization of the endocrine phenotype in these cells. They contrast with findings on NGF induction of neurite outgrowth in PC12 cells where PKC promotes differentiation, apparently by activating the fos/jun complex. Thus, we examined the role of c-fos in our model. Both histamine and bFGF induced c-fos gene expression transiently. To determine whether increased levels of c-fos oncoprotein were essential to the differentiation process, we used a hybrid arrest approach employing an innovative transfection technique applicable to primary culture systems. Transfection with plasmid pSVsof, producing antisense c-fos mRNA, reduced c-fos oncoprotein levels but did not diminish h...Continue Reading

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Citations

Feb 21, 1994·European Journal of Pharmacology·J A Maurer, D B McKay
Jan 1, 1992·Progress in Neuro-psychopharmacology & Biological Psychiatry·A L BoutillierB A Demeneix
Jan 16, 1995·European Journal of Pharmacology·J A MaurerD B McKay
Aug 11, 1992·Nucleic Acids Research·N KleyB R Seizinger
Oct 1, 1992·Journal of Neurochemistry·J A Maurer, D B McKay
Apr 30, 2011·Neurobiology of Disease·Sophorn ChipSven Wellmann

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