PMID: 6405801Jun 2, 1983Paper

Differentiation under the control of insulin of rat preadipocytes in primary culture. Isolation of homogeneous cellular fractions by gradient centrifugation

Biochimica Et Biophysica Acta
A M Gaben-CognevilleE Swierczewski

Abstract

Using a density gradient medium (Percoll) we succeeded in isolating homogeneous cell populations from the stromal-vascular fraction of the inguinal tissue of 3-day-old rats. In primary culture, in medium 199 supplemented with 10% fetal calf serum and 5.5 mM glucose, almost complete differentiation (90%) of these fractions was obtained for the first time in presence of a physiological concentration of insulin (10(-9) M). During the adipose conversion, insulin markedly enhanced the activities of glycerol-3-phosphate dehydrogenase and acid: CoA ligase. When VLDL and heparin were added with insulin to the medium, this effect was not potentiated. On the contrary, VLDL and heparin in presence of insulin increased the triglyceride content of the cells. With VLDL and heparin only, the biochemical and morphological characteristics of the cells were very similar to those observed in control culture. The heavier fraction was morphologically heterogeneous and did not undergo the adipose conversion to the same extent as the two lighter fractions. It was concluded that this model could be helpful in studying the proliferation and the differentiation of preadipocytes at an early stage of development.

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Citations

Apr 1, 1992·Journal of Cellular Biochemistry·J T Wright
Jan 1, 1987·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·G Serrero, D Mills
Aug 1, 1989·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·Y ReyneJ P Dulor
Oct 1, 1996·In Vitro Cellular & Developmental Biology. Animal·J L VierckM V Dodson
Aug 1, 1989·The Journal of Clinical Investigation·G ShillabeerD C Lau
Nov 23, 2012·Journal of Translational Medicine·J Christopher MizerAmit N Patel
Apr 28, 2009·Journal of Translational Medicine·Neil H RiordanBoris Minev
Dec 1, 1989·Journal of the American Geriatrics Society·C Remacle, N Hauser
May 1, 1992·Journal of Cellular Physiology·F GrégoireC Remacle
Nov 1, 1990·Experimental Cell Research·A M Gaben-CognevilleJ Mester
Jul 1, 1986·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·I DugailL Brigant
Jun 1, 1987·European Journal of Clinical Investigation·G Sypniewska, P Björntorp
Jun 27, 1986·Biochimica Et Biophysica Acta·R BrandesJ Bar-Tana

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