PMID: 6170647Oct 1, 1981Paper

Differing accessibility in chromatin of the antigenic sites of regions 1-58 and 63-125 of histone H2B

The Journal of Cell Biology
D Di Padua MathieuB D Stollar

Abstract

Experiments with antibodies induced by separated fragments 1-58 and 63-125 of H2B histone indicated that the 1-58 portion of the molecule is much more accessible in chromatin than is the 63-125 region. In immunoabsorption and immunoelectron microscopic assays with bovine and chicken chromatins, anti-1-58 antibodies reacted with sheared or unsheared chromatin both at low ionic strength (1 mM Tris-HCl) and in 0.14 M NaCl. Anti-63-125 antibodies were bound only weakly by chromatin at low ionic strength and not at all in 0.14 M NaCl. Antibodies to whole H2B showed intermediate reactivity with chromatin in both assays. In tests of immunofluorescence with unfixed calf liver nuclei in suspension, anti-1-58 caused nucleolar as well as nucleoplasmic fluorescence, whereas anti-63-125 did not lead to detectable fluorescence; anti-H2B showed intermediate staining intensity. In control experiments, anti-H1 antibody was bound by chromatin at low ionic strength but not in 0.14 M NaCl; anti-H3 antibody was bound poorly under either condition.

References

Apr 22, 1975·Biochemistry·D Goldblatt, M Bustin
Jan 1, 1978·Cold Spring Harbor Symposia on Quantitative Biology·S L McKnightO L Miller
Jan 1, 1977·Annual Review of Biochemistry·R D Kornberg
Jan 1, 1979·Annual Review of Biochemistry·I Isenberg
Feb 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·V C GordonV N Schumaker
Dec 20, 1977·FEBS Letters·D Absolom, M H Van Regenmortel
Sep 21, 1976·Biochemistry·R T Simpson, M Bustin
Jun 14, 1977·Biochemistry·L Feldman, B D Stollar
Oct 17, 1973·Nature: New Biology·M Bustin
Oct 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·H Weintraub, F Van Lente
Jan 1, 1972·Acta Endocrinologica. Supplementum·O L Miller, A H Bakken
Sep 1, 1972·Archives of Biochemistry and Biophysics·E W JohnsP L Riches
Jan 1, 1980·Annual Review of Biochemistry·J D McGhee, G Felsenfeld
Feb 29, 1980·Biochimica Et Biophysica Acta·D Goldblatt, M Bustin
Dec 25, 1980·Nature·J AllanF X Aviles
Nov 1, 1959·The Journal of Clinical Investigation·H HOLMAN, H R DEICHER

❮ Previous
Next ❯

Citations

Dec 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·J A Hardin, J O Thomas
Sep 1, 1987·European Journal of Biochemistry·V R RussanovaI G Pashev
Jan 1, 1986·Journal of Pharmaceutical Sciences·H P NguyenU W Kesselring
Mar 25, 1985·FEBS Letters·S MullerF A Bautz
Feb 20, 1986·Journal of Molecular Biology·J AllanC Crane-Robinson
Sep 14, 1983·Biochimica Et Biophysica Acta·S MullerM H Van Regenmortel

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.